Choi Byeong-Sun, Kim Sung Soon, Yun Haesun, Jang Dae Ho, Lee Joo-Shil
Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Seoul, Republic of Korea.
J Med Virol. 2007 Apr;79(4):426-30. doi: 10.1002/jmv.20826.
The distribution of HPV16 sequence variations differs geographically and specific HPV16 E6 and E7 variants might carry a high risk for development of invasive cervical cancer and cervical intraepithelial neoplasia in a given population. To investigate the genetic variation of HPV 16 E6 and E7 genes, genomic DNAs from 56 HPV16-infected commercial sex workers were extracted from their cervical swabs by using DNA isolation kit. The E6 and E7 coding region (34-880) with HPV16 E6/E7 specific PCR were amplified and analyzed by using the DNAstar software. At the nucleotide level, 26 variants of the HPV16 E6 and E7 genes were identified including 12 silent mutations. At the amino acid level, the isolates showed 14 variants including E6 Q14H, E6 D25E, E6 I27R, E6 H78Y, E6 L83V, and E7 N29S. The dominant HPV16 E6 and E7 variants were HPV16 E6 D25E (68%) and HPV16 E7 N29S (73%), respectively, which belong to Asian lineage. Although this study has some limitations such as a small sample size and not enough clinical data, these finding suggests that the distinctive distribution of HPV 16 As-variant E6 D25E and E7 N29S might be associated with geographical dependence rather than disease progression. Further study is needed to determine the clinical and biological effects of these variants.
人乳头瘤病毒16型（HPV16）序列变异的分布存在地域差异，特定的HPV16 E6和E7变异体在特定人群中可能具有发生浸润性宫颈癌和宫颈上皮内瘤变的高风险。为了研究HPV 16 E6和E7基因的遗传变异，使用DNA提取试剂盒从56名感染HPV16的商业性工作者的宫颈拭子中提取基因组DNA。通过HPV16 E6/E7特异性聚合酶链反应（PCR）扩增E6和E7编码区（34 - 880），并使用DNAstar软件进行分析。在核苷酸水平上，鉴定出26种HPV16 E6和E7基因变异体，包括12种沉默突变。在氨基酸水平上，分离株显示出14种变异体，包括E6 Q14H、E6 D25E、E6 I27R、E6 H78Y、E6 L83V和E7 N29S。HPV16 E6和E7的主要变异体分别是HPV16 E6 D25E（68%）和HPV16 E7 N29S（73%），它们属于亚洲谱系。尽管本研究存在一些局限性，如样本量小和临床数据不足，但这些发现表明，HPV 16亚洲变异体E6 D25E和E7 N29S的独特分布可能与地域依赖性有关，而非疾病进展。需要进一步研究来确定这些变异体的临床和生物学效应。
