Vaeteewoottacharn Kulthida, Jearanaikoon Patcharee, Ponglikitmongkol Mathurose
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Anticancer Res. 2003 Mar-Apr;23(2C):1927-31.
Infection with high-risk HPV16 is well associated with invasive cervical carcinomas. Variations in the E6 gene of HPV16 are shown to correlate with both the geographical areas and the oncogenicity. Here, we have characterized HPV16 DNA variants in the E6 and E7 coding regions of 31 Thai cervical squamous cell carcinomas. Five groups of E6 variants were identified. A mutation from T to G at nucleotide 178, leading to a change from aspartate to glutamate (D25E), was the most common variation accounting for 70%. Interestingly, 90% of these E6 (D25E) variants coincided with a specific type of E7 mutation, N29S. This is the first finding of coordinated change between E6 (D25E) and E7 (N29S) in HPV, which might indicate a specific variant for the Thai population. These comutations may not only represent an area-specific strain but may also be valuable for studying the virus and developing a suitable vaccine in Thailand.
高危型人乳头瘤病毒16型（HPV16）感染与浸润性宫颈癌密切相关。研究表明，HPV16 E6基因的变异与地理区域和致癌性均相关。在此，我们对31例泰国宫颈鳞状细胞癌的E6和E7编码区中的HPV16 DNA变异进行了特征分析。共鉴定出五组E6变异。核苷酸178处由T突变为G，导致天冬氨酸变为谷氨酸（D25E），这是最常见的变异，占70%。有趣的是，这些E6（D25E）变异中有90%与一种特定类型的E7突变（N29S）一致。这是首次发现HPV中E6（D25E）和E7（N29S）之间的协同变化，这可能表明这是泰国人群特有的变异。这些共突变不仅可能代表一种区域特异性毒株，而且对于在泰国研究该病毒和开发合适的疫苗也可能具有重要价值。
