Lyte M, Ernst S
Department of Biological Sciences Mankato State University, MN 56002.
Life Sci. 1992;50(3):203-12. doi: 10.1016/0024-3205(92)90273-r.
The addition of various catecholamines to cultures of gram negative bacteria resulted in dramatic increases in growth. The ability of norepinephrine, epinephrine, dopamine and dopa to enhance the growth of Escherichia coli, Yersinia enterocolitica and Pseudomonas aeruginosa was observed to be dependent on the bacterium employed with each strain showing marked preference for one or more of the catecholamines. Catecholamine induced increases in growth were confirmed by one or more of the following methods: uptake of tritiated thymidine into newly synthesized DNA, changes in optical density or pour plate analysis. None of the catecholamine metabolites resulting from either oxidative deamination or catechol-O-methylation were able to effect similar increases in bacterial growth as compared to either norepinephrine, epinephrine or dopamine. Norepinephrine was consistently observed to effect the greatest increase in bacterial growth for all strains tested.
在革兰氏阴性菌培养物中添加各种儿茶酚胺会导致生长显著增加。观察到去甲肾上腺素、肾上腺素、多巴胺和多巴增强大肠杆菌、小肠结肠炎耶尔森菌和铜绿假单胞菌生长的能力取决于所使用的细菌，每种菌株对一种或多种儿茶酚胺表现出明显的偏好。儿茶酚胺诱导的生长增加通过以下一种或多种方法得到证实：将氚标记的胸腺嘧啶核苷摄取到新合成的DNA中、光密度变化或倾注平板分析。与去甲肾上腺素、肾上腺素或多巴胺相比，氧化脱氨或儿茶酚-O-甲基化产生的儿茶酚胺代谢产物均不能使细菌生长产生类似的增加。对于所有测试菌株，始终观察到去甲肾上腺素对细菌生长的增加作用最大。
