Hayashi M
Department of Obstetrics and Gynecology, Koshigaya Hospital, Dokkyo University School of Medicine, 2-1-50, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, Japan.
J Med. 1999;30(3-4):191-8.
Live platelets were used to determine whether platelet count, volume, aggregation, and the amount of secreted adenosine triphosphate (ATP) change after laparotomy. Peripheral blood was collected from 32 gynecological patients before, one day, and two weeks after laparotomy. The platelet count was significantly lower (p < 0.01) in patients one day after laparotomy than before the operation. The mean platelet volume was significantly lower in patients two weeks after laparotomy than before (p < 0.01) and one day after laparotomy (p < 0.01). Platelet aggregation and ATP secretion were investigated using a lumiaggregometer. Platelet aggregation upon induction by 5 microM adenosine diphosphate (ADP) was significantly higher (p < 0.05) in patients two weeks after laparotomy than before laparotomy. Although the amount of secreted ATP upon induction by 5 microM ADP did not differ between before and after laparotomy, the amount of secreted ATP induced by 5 microg/mL collagen was significantly lower (p < 0.05) in patients one day after laparotomy than before the operation. This discrepancy may have been caused by a difference in the action of the two reagents.
使用活血小板来确定剖腹手术后血小板计数、体积、聚集以及分泌的三磷酸腺苷（ATP）量是否发生变化。从32例妇科患者剖腹手术前、术后1天和术后2周采集外周血。剖腹手术后1天患者的血小板计数显著低于手术前（p < 0.01）。剖腹手术后2周患者的平均血小板体积显著低于手术前（p < 0.01）和剖腹手术后1天（p < 0.01）。使用光聚集仪研究血小板聚集和ATP分泌。剖腹手术后2周患者由5微摩尔二磷酸腺苷（ADP）诱导的血小板聚集显著高于剖腹手术前（p < 0.05）。虽然由5微摩尔ADP诱导的ATP分泌量在剖腹手术前后没有差异，但剖腹手术后1天患者由5微克/毫升胶原诱导的ATP分泌量显著低于手术前（p < 0.05）。这种差异可能是由两种试剂作用的不同所导致的。
