Sabet Negar Shafiei, Subramaniam Geetha, Navaratnam Parasakthi, Sekaran Shamala Devi
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Int J Antimicrob Agents. 2007 May;29(5):582-5. doi: 10.1016/j.ijantimicag.2006.12.017. Epub 2007 Feb 20.
A triplex real-time polymerase chain reaction (PCR) assay was used for the simultaneous detection of mecA (methicillin resistance), ermA (erythromycin resistance) and femA (Staphylococcus aureus identification) genes in a single assay. Among 93 clinical S. aureus hospital isolates, there were 48 methicillin-resistant S. aureus (MRSA) and 45 methicillin-sensitive S. aureus (MSSA) isolates. Screening the isolates using the triplex real-time PCR assay, the mecA, ermA and femA genes were detected in all MRSA isolates. The triplex real-time PCR assay was completed within 3h and is a useful genotypic method for detecting the resistance determinants as well as for the identification of S. aureus isolates. These findings will assist the clinical laboratory in identifying these resistance genes and S. aureus rapidly, thus benefiting patient therapy. This study represents a valuable source of information for researchers to study the local antibiotic resistance pattern, which can increase our knowledge of the antibiotic resistance profile, using real-time PCR technology.
采用三重实时聚合酶链反应（PCR）分析法在一次检测中同时检测mecA（耐甲氧西林）、ermA（耐红霉素）和femA（金黄色葡萄球菌鉴定）基因。在93株临床分离的医院金黄色葡萄球菌中，有48株耐甲氧西林金黄色葡萄球菌（MRSA）和45株甲氧西林敏感金黄色葡萄球菌（MSSA）。使用三重实时PCR分析法对分离株进行筛查，在所有MRSA分离株中均检测到mecA、ermA和femA基因。三重实时PCR分析法在3小时内完成，是一种用于检测耐药决定因素以及鉴定金黄色葡萄球菌分离株的有用的基因分型方法。这些发现将有助于临床实验室快速鉴定这些耐药基因和金黄色葡萄球菌，从而使患者治疗受益。本研究为研究人员研究当地抗生素耐药模式提供了有价值的信息来源，利用实时PCR技术可以增加我们对抗生素耐药谱的了解。
