Jung Chaewon, Kim Gyeohoon, Song Mun-Bum, Kim Min-Gon
Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science & Technology (GIST), 123 Cheomdan-gwagiro, Gwangju 61005, Republic of Korea.
GMD Biotech., 416, Research Institute of University Industry Cooperation, Gwangju Institute of Science & Technology (GIST), 123 Cheomdan-gwagiro, Gwangju 61005, Republic of Korea.
ACS Omega. 2025 May 15;10(20):20427-20434. doi: 10.1021/acsomega.5c00573. eCollection 2025 May 27.
Nucleic acid extraction from clinical specimens is essential for accurate molecular diagnostic assays. However, conventional extraction methods are often slow, equipment-dependent, and require trained personnel, limiting the point-of-care applicability. We introduce a novel silica-based pipet tip column that enables rapid nucleic acid extraction from clinical samples in less than 3 min. The pipet tip column has a large surface area for effective nucleic acid extraction using chaotropic salts. The integrated disposable 1 mL syringe eliminates the need for a pipet. Sample application is achieved by simply pushing and pulling the syringe, requiring no external power or specialized equipment. The pipet tip column performance was validated using standard influenza A and B, severe acute respiratory syndrome coronavirus-2, and Staphylococcus aureus samples, achieving high performance even at low concentrations (1 PFU/mL or 1 TCID/mL). Our approach demonstrated a 90-110% nucleic acid recovery rate, comparable to that of traditional methods. Pipette tip-column nucleic acid extraction was successful for nasopharyngeal and saliva samples, including those in loop-mediated isothermal amplification assays. The pipet tip column provides a rapid, cost-effective, reliable, and portable solution for on-site molecular diagnostics. Additionally, nonexpert users can perform nucleic acid extraction without sample transportation or storage, reducing contamination risks and operational costs.
从临床样本中提取核酸对于准确的分子诊断检测至关重要。然而，传统的提取方法往往速度慢、依赖设备且需要训练有素的人员，限制了即时检测的适用性。我们推出了一种新型的基于硅胶的移液器吸头柱，可在不到3分钟的时间内从临床样本中快速提取核酸。该移液器吸头柱具有较大的表面积，可使用离液盐有效地提取核酸。集成的一次性1 mL注射器无需移液器。通过简单地推拉注射器即可实现样品加样，无需外部电源或专用设备。使用甲型和乙型流感标准毒株、严重急性呼吸综合征冠状病毒2以及金黄色葡萄球菌样本对移液器吸头柱的性能进行了验证，即使在低浓度（1 PFU/mL或1 TCID/mL）下也能实现高性能。我们的方法显示核酸回收率为90%至110%，与传统方法相当。移液器吸头柱核酸提取对于鼻咽和唾液样本（包括那些用于环介导等温扩增检测的样本）是成功的。该移液器吸头柱为现场分子诊断提供了一种快速、经济高效、可靠且便携的解决方案。此外，非专业用户无需运输或储存样本即可进行核酸提取，降低了污染风险和运营成本。
