Biswanger Caroline, Davis Laura, Roberts Rebecca A
Department of Biology, Ursinus College, Collegeville, Pennsylvania 19426, USA.
In Vitro Cell Dev Biol Anim. 2006 Nov-Dec;42(10):294-7. doi: 10.1290/0608050.1.
Control of the cellular environment is a principal attribute of in vitro cell cultures. Unintentional exposure to environmental compounds can adversely affect cultures and, therefore, experimental results. Estrogenic compounds arising from common plastic ware have been found during cell culture. One such compound, the environmental endocrine disrupting chemical bisphenol A, can bind to estrogen receptors and effect cellular changes. We monitored bisphenol A concentrations in culture dishes from six different manufacturers under typical cell-culture conditions. With the use of a gas chromatography mass-spectrometry assay we determined that bisphenol A contamination from the culture dishes did not occur. These findings will allow scientists concerned about possible effects of bisphenol A on their culture systems to choose appropriate plastic ware.
控制细胞环境是体外细胞培养的一个主要特性。意外接触环境化合物会对培养物产生不利影响，进而影响实验结果。在细胞培养过程中发现，常见塑料制品会产生雌激素类化合物。其中一种化合物，即环境内分泌干扰化学物质双酚A，可与雌激素受体结合并引起细胞变化。我们在典型的细胞培养条件下，监测了六个不同制造商生产的培养皿中双酚A的浓度。通过气相色谱-质谱分析法，我们确定培养皿中不存在双酚A污染。这些发现将使担心双酚A对其培养系统可能产生影响的科学家能够选择合适的塑料制品。
