Bhadra Bhaskar, Sreenivas Rao R, Naveen Kumar N, Chaturvedi Preeti, Sarkar Partha K, Shivaji S
Centre for Cellular and Molecular Biology, Hyderabad, India.
FEMS Yeast Res. 2007 Jun;7(4):579-84. doi: 10.1111/j.1567-1364.2007.00215.x. Epub 2007 Feb 22.
The ascogenous yeast YS16T was isolated from a decaying papaya fruit. Phenotypic traits such as multilateral budding, spheroidal or elongate shape, pseudohyphae formation, asci with one or more ascospores, ability to ferment d-glucose, inability to assimilate nitrate and the presence of Q7 ubiquinone suggest its affiliation to the genus Pichia. The nearest phylogenetic neighbor, based on D1/D2 domain sequence of the 26S rRNA gene and ITS region sequence, was identified as Issatchenkia orientalis (NRRL Y-5396T, a synonym of Pichia kudriavzevii) with similarities of 98.2% and 97% respectively. In addition to the difference in the D1/D2 and ITS region sequence, YS16T differs from I. orientalis with respect to a number of phenotypic traits. However, in the phylogenetic analysis, YS16T showed close relatedness to the P. membranifaciens clade. Thus, it is proposed to assign the status of a new species to YS16T, for which the name P. cecembensis sp. nov. is proposed. The type strain of P. cecembensis sp. nov. is YS16T (=NRRL Y-27985T=JCM 13873T=CBS 10445T).
产囊酵母YS16T是从腐烂的木瓜果实中分离得到的。其表型特征包括多边芽殖、球形或椭圆形、假菌丝形成、具一个或多个子囊孢子的子囊、发酵d-葡萄糖的能力、不能同化硝酸盐以及存在泛醌Q7,这些表明它隶属于毕赤酵母属。基于26S rRNA基因的D1/D2结构域序列和ITS区域序列,与之最近的系统发育邻种被鉴定为东方伊萨酵母(NRRL Y-5396T,库德里阿兹威毕赤酵母的同义词),相似度分别为98.2%和97%。除了D1/D2和ITS区域序列存在差异外,YS16T在一些表型特征上也与东方伊萨酵母不同。然而,在系统发育分析中,YS16T显示出与膜醭毕赤酵母分支密切相关。因此,建议将YS16T赋予一个新物种的地位,为此提出新名称毕赤酵母cecembensis sp. nov.。毕赤酵母cecembensis sp. nov.的模式菌株是YS16T(=NRRL Y-27985T=JCM 13873T=CBS 10445T)。