Pruski A M, Dixon D R
National Oceanography Centre, University of Southampton, Waterfront Campus, Southampton SO14 3ZH, United Kingdom.
Mar Environ Res. 2007 Aug;64(2):209-24. doi: 10.1016/j.marenvres.2007.01.003. Epub 2007 Jan 17.
We previously reported evidence of increased levels of DNA damage in the hydrothermal mussel Bathymodiolus azoricus, which suggested that the species was not fully resistant to the natural toxicity of its deep-sea vent environment. In the present study, HSP70 was used as a biomarker of sub-cellular stress. Differences in HSP70 expression pattern were observed between vent sites, typified by different depths/toxicity profiles, and between different mussel tissue types. A comparison of specimens collected by remote operated vehicle (ROV) and acoustically-operated cages showed that less stress (as indicated by changes in HSP70 levels) was induced by the faster cage recovery method. Therefore alternatives to ROV collection should be considered when planning experiments involving live deep sea organisms. Significantly, a positive correlation was found between the levels of DNA strand breakage, as measured using the Comet assay, and HSP70 expression pattern; evidence was also obtained for the constitutive expression of at least one HSP isoform which was located within the cell nucleus.
我们之前报道过有关热液贻贝阿氏深海贻贝（Bathymodiolus azoricus）DNA损伤水平升高的证据，这表明该物种对其深海热液喷口环境的自然毒性并未完全具备抗性。在本研究中，热休克蛋白70（HSP70）被用作亚细胞应激的生物标志物。在以不同深度/毒性特征为代表的喷口位点之间以及不同贻贝组织类型之间，观察到了HSP70表达模式的差异。对通过遥控潜水器（ROV）采集的标本和声学操作网箱采集的标本进行比较发现，更快的网箱回收方法所诱导的应激（以HSP70水平的变化表示）较小。因此，在规划涉及活深海生物的实验时，应考虑采用替代ROV采集的方法。值得注意的是，使用彗星试验测得的DNA链断裂水平与HSP70表达模式之间存在正相关；还获得了证据，证明至少有一种HSP异构体在细胞核内组成性表达。
