Löttrich Mathias, Mawrin Christian, Chamaon Kathrin, Kirches Elmar, Dietzmann Knut, Freigang Bernd
Department of Neuropathology, Otto-von-Guericke University, Magdeburg, Germany.
Pathol Res Pract. 2007;203(4):245-9. doi: 10.1016/j.prp.2006.12.007. Epub 2007 Feb 20.
Expression of the transforming growth factor-beta (TGF-beta) protein family in the peripheral nervous system is well established, but the role of their cognate receptors TGF-beta receptor type 1 (R1) and type 2 (R2) has been less well studied. TGF-beta plays an essential role in Schwann cell proliferation and differentiation, and is involved in neurotrophic effects of several neurotrophic substances. TGF-beta is also expressed in benign peripheral nervous system tumors such as vestibular schwannomas. In the present study, we aimed to detect TGF-beta R1 and R2 in a total of 40 sporadic vestibular schwannomas using immunohistochemistry, and correlated the findings to essential clinicopathologic data. TGF-beta, TGF-beta R1, and TGF-beta R2 mRNA was further analyzed by RT-PCR in six vestibular schwannomas. TGF-beta R1 immunoexpression was found in about 95% of the tumors. TGF-beta R1 was equally present in Antoni A and Antoni B areas of the tumors. TGF-beta R2 was found immunohistochemically in 77%. In addition, all tumors showed strong expression of TGF-beta. No correlation between TGF-beta R1 or R2 expression and clinicopathologic parameters such as age, sex, clinical symptoms, growth pattern, and proliferation acitivity as measured by Ki-67 (MIB-1) staining was found. Moreover, all schwannomas studied contained TGF-beta, TGF-beta R1, and TGF-beta R2 mRNA. Therefore, the TGF-beta/TGF-beta R1 and -R2 system is present in human schwannomas, but its biologic role for tumor development and growth remains unclear.
转化生长因子-β（TGF-β）蛋白家族在周围神经系统中的表达已得到充分证实，但其同源受体转化生长因子-β受体1型（R1）和2型（R2）的作用研究较少。TGF-β在雪旺细胞增殖和分化中起重要作用，并参与多种神经营养物质的神经营养作用。TGF-β也表达于良性周围神经系统肿瘤，如前庭神经鞘瘤。在本研究中，我们旨在通过免疫组织化学检测40例散发性前庭神经鞘瘤中TGF-β R1和R2，并将结果与重要的临床病理数据相关联。通过RT-PCR对6例前庭神经鞘瘤进一步分析TGF-β、TGF-β R1和TGF-β R2 mRNA。在约95%的肿瘤中发现TGF-β R1免疫表达。TGF-β R1在肿瘤的Antoni A区和Antoni B区中均有表达。免疫组织化学检测发现77%的肿瘤中有TGF-β R2。此外，所有肿瘤均显示TGF-β的强表达。未发现TGF-β R1或R2表达与年龄、性别、临床症状、生长模式以及通过Ki-67（MIB-1）染色测量的增殖活性等临床病理参数之间存在相关性。此外，所有研究的神经鞘瘤均含有TGF-β、TGF-β R1和TGF-β R2 mRNA。因此，TGF-β/TGF-β R1和-R2系统存在于人类神经鞘瘤中，但其在肿瘤发生和生长中的生物学作用仍不清楚。
