Hrycek Antoni, Kusmierz Dariusz, Dybała Tomasz, Swiatkowska Longina
Department of Internal, Autoimmune and Metabolic Diseases, Medical University of Silesia, ul Medyków 14, Katowice, Poland.
Autoimmunity. 2007 Feb;40(1):23-30. doi: 10.1080/08916930601168093.
The objective of this study was to determine the expression of transforming growth factor-beta1 messenger RNA (TGF-beta1 mRNA) and the expression of mRNA for TGF-beta receptors (TGF-beta Rs mRNA) in whole peripheral blood of consecutive (treated from several months to several years) systemic lupus erythematosus (SLE) patients (21 women). A further aim of this study was to evaluate the association between expression of the above mentioned parameters in relation to the form of applied therapy (9 patients treated with quinagolide and 12 with quinagolide plus prednisone, azathioprine or cyclosporine A). The control group consisted of 15 healthy women. Most of the patients had mild SLE with SLE disease activity index (SLEDAI) score < 10 at time when blood samples were collected. Laboratory measurements included real-time polymerase chain reaction (RT-QPCR). The expression levels of TGF-beta1 mRNA and mRNA for TGF-beta RII and RIII were significantly lower in patients whereas the expression level of TGF-beta RI was statistically significantly higher in SLE patients than in the controls. A very high positive correlation between TGF-beta1 mRNA expression and expression levels of TGF-beta Rs mRNA was found. In compared subgroups selected according to the form of the applied therapy no statistically significant differences were observed. We conclude that the TGF-beta signaling pathway can be altered in circulating leukocytes derived from treated patients with SLE and that the assumed forms of the applied therapy in the group of patients under consideration are accompanied by similarity in the expression level of transcripts for TGF-beta1 and TGF-beta Rs determined in whole blood. In our investigations, we cannot exclude the influence of the disease itself on the obtained results.
本研究的目的是测定连续（治疗数月至数年）的系统性红斑狼疮（SLE）患者（21名女性）外周血中转化生长因子β1信使核糖核酸（TGF-β1 mRNA）的表达以及TGF-β受体的信使核糖核酸（TGF-β Rs mRNA）的表达。本研究的另一个目的是评估上述参数的表达与所应用治疗方式之间的关联（9名患者接受喹那高利治疗，12名患者接受喹那高利联合泼尼松、硫唑嘌呤或环孢素A治疗）。对照组由15名健康女性组成。大多数患者在采集血样时患有轻度SLE，SLE疾病活动指数（SLEDAI）评分<10。实验室检测包括实时聚合酶链反应（RT-QPCR）。患者中TGF-β1 mRNA以及TGF-β RII和RIII的信使核糖核酸表达水平显著降低，而SLE患者中TGF-β RI的表达水平在统计学上显著高于对照组。发现TGF-β1 mRNA表达与TGF-β Rs mRNA表达水平之间存在非常高的正相关性。在根据所应用治疗方式选择的比较亚组中，未观察到统计学上的显著差异。我们得出结论，SLE治疗患者循环白细胞中的TGF-β信号通路可能发生改变，并且在所考虑的患者组中，所应用的假定治疗方式伴随着全血中TGF-β1和TGF-β Rs转录本表达水平的相似性。在我们的研究中，我们不能排除疾病本身对所得结果的影响。
