从类风湿性滑膜组织中产生并鉴定两种单克隆自缔合IgG类风湿因子。
Generation and characterization of two monoclonal self-associating IgG rheumatoid factors from a rheumatoid synovium.
作者信息
Lu E W, Deftos M, Tighe H, Carson D A, Chen P P
机构信息
Department of Medicine, University of California, San Diego 92093.
出版信息
Arthritis Rheum. 1992 Jan;35(1):101-5. doi: 10.1002/art.1780350115.
OBJECTIVE
To study IgG rheumatoid factor (RF) from rheumatoid synovium.
METHODS
We fused the K6H6/B5 human-mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF-secreting hybridomas.
RESULTS
The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium.
CONCLUSION
IgG-RF-secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF.
目的
研究类风湿滑膜中的IgG类风湿因子（RF）。
方法
将K6H6/B5人鼠异源杂交瘤与未受刺激的类风湿滑膜B细胞融合，以产生分泌IgG-RF的杂交瘤。
结果
来自2个此类杂交瘤的RF特异性结合人IgG的Fc片段并自身缔合形成免疫复合物。此类免疫复合物是类风湿滑膜中致病性IgG-RF的主要特征。
结论
已获得分泌IgG-RF的杂交瘤。分析可能揭示诱导IgG-RF的潜在机制。
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