Randen I, Thompson K M, Thorpe S J, Førre O, Natvig J B
Institute of Immunology and Rheumatology, University of Oslo, Norway.
Scand J Immunol. 1993 Jun;37(6):668-72. doi: 10.1111/j.1365-3083.1993.tb01681.x.
We have established IgG rheumatoid factor (RF)-secreting hybridoma cell lines from the synovial tissues of two patients (TS and SJ) with rheumatoid arthritis (RA) and one (KL) with the polyarticular form of juvenile rheumatoid arthritis (JRA). The IgG RF bind human and rabbit IgG and all except one form intracellular complement-fixing complexes indicative of a self-association process. The possibility of IgG RF for self association and immune complex formation is a feature thought to be important for the inflammatory processes in RA. Of the IgG RF-secreting cell lines established, three clones from patient SJ and one from patient KL are of the IgG1 kappa isotype while five clones from patient TS are of the IgG2 lambda isotype.
我们从两名类风湿关节炎（RA）患者（TS和SJ）以及一名多关节型幼年类风湿关节炎（JRA）患者（KL）的滑膜组织中建立了分泌IgG类风湿因子（RF）的杂交瘤细胞系。IgG RF可结合人和兔IgG，除一种外，所有形式均形成细胞内补体固定复合物，这表明存在自缔合过程。IgG RF自缔合和形成免疫复合物的可能性被认为是RA炎症过程中的一个重要特征。在所建立的分泌IgG RF的细胞系中，来自患者SJ的三个克隆和来自患者KL的一个克隆为IgG1 κ同种型，而来自患者TS的五个克隆为IgG2 λ同种型。
