Poomsawat Sopee, Punyasingh Jirapa
Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Yothi Street, Bangkok 10400, Thailand.
J Mol Histol. 2007 Mar;38(1):103-9. doi: 10.1007/s10735-007-9082-9. Epub 2007 Feb 22.
Calcifying epithelial odontogenic tumor (CEOT) is a rare benign odontogenic tumor. A case of CEOT in a 25-year-old female is presented here. Histologically, the case showed sheets of polyhedral epithelial cells with deep eosinophilic cytoplasm and prominent nuclei. Nuclear pleomorphism and hyperchromatism were evident. Globules of amyloid-like material among the tumor cells were prominent. Also found was a small area demonstrating a cribriform pattern. Immunohistochemical studies with antibodies against basement membrane proteins (laminins 1 and 5, collagen type IV and fibronectin), pan-cytokeratins AE1/AE3, vimentin, S-100 protein and CD 1a were performed. Tumor cells expressed laminins 1 and 5, fibronectin, cytokeratins and vimentin. The amyloid-like material was not reactive to all antibodies examined. A number of dendritic cells among sheets of tumor cells were revealed with strong staining for S-100 protein and CD 1a. These dendritic cells are likely to be Langerhans cells. Hence, immunohistochemistry is a useful method to study the variant of CEOT.
钙化上皮性牙源性肿瘤（CEOT）是一种罕见的良性牙源性肿瘤。本文报告一例25岁女性的CEOT病例。组织学上，该病例显示多角形上皮细胞成片，胞质嗜酸性深，细胞核突出。核异形性和核深染明显。肿瘤细胞间淀粉样物质小球突出。还发现一个小区域呈筛状结构。用抗基底膜蛋白（层粘连蛋白1和5、IV型胶原和纤连蛋白）、泛细胞角蛋白AE1/AE3、波形蛋白、S-100蛋白和CD1a的抗体进行免疫组织化学研究。肿瘤细胞表达层粘连蛋白1和5、纤连蛋白、细胞角蛋白和波形蛋白。淀粉样物质对所有检测抗体均无反应。在成片的肿瘤细胞中发现许多树突状细胞，S-100蛋白和CD1a染色强。这些树突状细胞可能是朗格汉斯细胞。因此，免疫组织化学是研究CEOT变异型的有用方法。
