Poomsawat Sopee, Punyasingh Jirapa, Vejchapipat Paisarn
Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Nov;104(5):666-75. doi: 10.1016/j.tripleo.2006.08.025. Epub 2006 Dec 5.
The objective was to characterize the expression of BMCs (laminins 1 and 5, collagen type IV, and fibronectin) in ameloblastomas, calcifying cystic odontogenic tumors (CCOTs), and adenomatoid odontogenic tumors (AOTs).
BMCs were analyzed in 14 ameloblastomas, 7 CCOTs, and 7 AOTs using immunohistochemistry.
In normal oral mucosa, linear deposits of these proteins were found at the epithelial-mesenchymal junction, but not in epithelial cytoplasm. In all tumors studied, linear deposits of all proteins were found at the epithelial-mesenchymal junction; laminin 1 was expressed in all tumor cells, regardless of cell types. For CCOTs, laminin 5 was found faintly in suprabasal cells, but expressed strongly in ghost cells. For AOTs, laminin 5 strongly decorated tumor cells adjacent to mineralization.
Laminin 1 may be a marker for odontogenic epithelium. Additionally, laminin 5 may be involved in ghost cell formation and initiation of calcification.
本研究旨在明确牙釉质瘤、牙源性钙化囊性肿瘤（CCOT）及牙源性腺样瘤（AOT）中基底膜成分（层粘连蛋白1和5、IV型胶原及纤连蛋白）的表达情况。
采用免疫组织化学方法对14例牙釉质瘤、7例CCOT及7例AOT中的基底膜成分进行分析。
在正常口腔黏膜中，这些蛋白呈线性沉积于上皮-间充质交界处，而非上皮细胞质中。在所研究的所有肿瘤中，所有蛋白均呈线性沉积于上皮-间充质交界处；层粘连蛋白1在所有肿瘤细胞中均有表达，与细胞类型无关。对于CCOT，层粘连蛋白5在上皮基底上层细胞中表达较弱，但在影细胞中表达较强。对于AOT，层粘连蛋白5在矿化附近的肿瘤细胞中呈强阳性表达。
层粘连蛋白1可能是牙源性上皮的标志物。此外，层粘连蛋白5可能参与影细胞形成及钙化起始过程。
