Wang Jin, Yu Han-Qing
School of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Appl Microbiol Biotechnol. 2007 Jun;75(4):871-8. doi: 10.1007/s00253-007-0870-7. Epub 2007 Feb 21.
The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and (NH4)2SO4 concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and (NH4)2SO4 of 3.2 g/l.
研究了在分批培养中，不同葡萄糖和硫酸铵浓度下，真养产碱杆菌ATCC 17699合成聚羟基丁酸酯（PHB）和胞外聚合物（EPS）的情况。真养产碱杆菌合成EPS的过程与细胞生长紧密相关，而PHB仅在缺氮和细胞生长受限的条件下合成。实验结果表明，PHB的比生产速率与比细胞生长速率和EPS生产速率均呈指数相关。此外，在氮限制条件下，PHB被观察到是真养产碱杆菌主要的碳和能源储存物质。另外，基于中心复合设计进行实验以优化分批培养，从而获得高PHB产量。在葡萄糖浓度为38.2 g/l和硫酸铵浓度为3.2 g/l时，基于葡萄糖的PHB产量达到最大值0.34 g/g。
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