Jendrossek Dieter
Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70550 Stuttgart, Germany.
Appl Microbiol Biotechnol. 2007 Apr;74(6):1186-96. doi: 10.1007/s00253-007-0860-9. Epub 2007 Feb 21.
An extensive amount of knowledge on biochemistry of poly(3-hydroxyalkanoic acid) (PHA) synthesis and on its biodegradation has accumulated during the last two decades. Numerous genes encoding enzymes involved in the formation of PHA and in PHA degradation (PHA depolymerases) were cloned and characterized from many microorganisms. A large variety of methods exists for determination of PHA depolymerase activity and for preparation of the polymeric substrate (PHA). Unfortunately, results obtained with these different methods cannot be compared directly because they highly depend on the assay method applied and on the history of PHA granules preparation. In this contribution, the peculiarities, advantages, disadvantages and limitations of existing PHA depolymerase assay methods are described.
在过去二十年中，关于聚（3-羟基链烷酸）（PHA）合成的生物化学及其生物降解方面积累了大量知识。从许多微生物中克隆并鉴定了众多编码参与PHA形成和PHA降解（PHA解聚酶）的酶的基因。存在多种用于测定PHA解聚酶活性和制备聚合物底物（PHA）的方法。不幸的是，用这些不同方法获得的结果无法直接比较，因为它们高度依赖于所应用的测定方法以及PHA颗粒制备的历史。在本论文中，描述了现有PHA解聚酶测定方法的特点、优点、缺点和局限性。
