Campbell Katrina, Fodey Terence, Flint Jonathan, Danks Christopher, Danaher Martin, O'Keeffe Michael, Kennedy D Glenn, Elliott Christopher
Institute of Agri-Food and Land Use, Queen's University, David Keir Building, Stranmillis Road, Belfast BT9 5AG, UK.
J Agric Food Chem. 2007 Mar 21;55(6):2497-503. doi: 10.1021/jf062811d. Epub 2007 Feb 24.
Concentrations of the coccidiostat nicarbazin as low as 2 mg/kg in feed can result in violative drug residues arising in poultry liver. A lateral flow device (LFD) was developed for the detection of contaminating concentrations of nicarbazin following solvent extraction of poultry feeds. Test results, as determined by both visual and instrumental measurement, are available within minutes. For 22 feed samples, nicarbazin-free and fortified at 2 mg/kg, the % relative inhibition ranged from 0 to 45% and from 53 to 85%, respectively. Nicarbazin contamination at the critical concentration (2 mg/kg) can be determined in all cases providing the sampling is representative. A wide range of feed samples taken at a mill that incorporated nicarbazin into poultry feed were analyzed. Data generated for these samples by both the LFDs and a mass spectrometric method were compared, and a significant correlation was achieved.
