Xu D-Y, Tang Y-D, Liu S-X, Liu J
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu, China.
J Laryngol Otol. 2007 Aug;121(8):721-4. doi: 10.1017/S0022215107005993. Epub 2007 Feb 26.
Endothelin 1 is a vasoconstrictive peptide with many biological functions. To investigate the distribution of endothelin 1 in guinea pig cochlear lateral wall and the significance of endothelin 1 in maintaining cochlear homeostasis, the immunohistochemistry avidin biotin complex method was applied by using rabbit anti-endothelin 1 polyclonal antibody as primary antibody. Endothelin-1-like activities were detected in the marginal cells, spiral prominence epithelial cells, outer sulcus cells, stria vascularis capillaries, basal cells and spiral ligament fibrocytes. These results suggest that endothelin 1 may play an important role in maintaining cochlear homeostasis.
内皮素-1是一种具有多种生物学功能的血管收缩肽。为了研究内皮素-1在豚鼠耳蜗外侧壁的分布以及内皮素-1在维持耳蜗内环境稳定中的意义，以兔抗内皮素-1多克隆抗体为一抗，采用免疫组织化学抗生物素蛋白-生物素复合物法。在边缘细胞、螺旋隆起上皮细胞、外沟细胞、血管纹毛细血管、基底细胞和螺旋韧带纤维细胞中检测到内皮素-1样活性。这些结果表明，内皮素-1可能在维持耳蜗内环境稳定中起重要作用。
