Xu Ding-Yuan, Wu Bo, Li Zhe-Qin, Wang Qiu-Ping, Zhang Yong, Xue Fei, Ji Jun-Feng
Department of Otorhinolaryngology, Jinling Hospital, Clinical School of Nanjing, University, 305 East Zhongshan Road, Nanjing 210002, China.
Int J Pediatr Otorhinolaryngol. 2010 Feb;74(2):164-7. doi: 10.1016/j.ijporl.2009.11.003. Epub 2009 Dec 2.
Endothelin 1 has many biological activities including regulating the functions of auditory system. The present study aimed to investigate the expression of the endothelin receptors in spiral ganglion neurons and its significance in the auditory transmission.
The cochleae of healthy guinea pigs were fixed, decalcified, embedded in paraffin and serially sectioned. The expression of endothelin receptor subunits, ET receptor A (ET-A) and ET receptor B (ET-B), was examined in the spiral ganglion neurons of guinea pig using immunohistochemical technique.
Different degrees of ET-A and ET-B positive reactivity appeared in all spiral ganglion neurons from the basal turn to the apical turn.
These findings support the suggestion that endothelin via the endothelin receptor may play a physiological role in the spiral ganglion neurons.
内皮素1具有多种生物学活性，包括调节听觉系统功能。本研究旨在探讨螺旋神经节神经元中内皮素受体的表达及其在听觉传导中的意义。
将健康豚鼠的耳蜗固定、脱钙、石蜡包埋并连续切片。采用免疫组织化学技术检测豚鼠螺旋神经节神经元中内皮素受体亚单位，即内皮素A受体（ET-A）和内皮素B受体（ET-B）的表达。
从蜗底到蜗顶的所有螺旋神经节神经元均出现不同程度的ET-A和ET-B阳性反应。
这些发现支持了内皮素通过内皮素受体可能在螺旋神经节神经元中发挥生理作用的观点。
