Peng Donghai, Chen Shouwen, Ruan Lifang, Li Lin, Yu Ziniu, Sun Ming
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Food Chem Toxicol. 2007 Jul;45(7):1179-85. doi: 10.1016/j.fct.2006.12.026. Epub 2007 Jan 11.
The aim of this study was to evaluate the toxicology safety of a genetically modified (GM) Bacillus thuringiensis with vegetative insecticidal protein (VIP) gene. Acute and subacute toxicity studies by using its powder preparation were conducted in Wistar rats. The result of the acute study showed the no-observable-adverse-effect level (NOAEL) of this GM B. thuringiensis powder preparation was greater than 5000 mg/kg body weight (BW). In the subacute study, the data analysis of body weight gain, food and water consumptions, clinical observations, haematology, serum biochemistry, organ weight ratios and histopathological findings did not show significant differences between control and treated groups. These results proved the NOAEL of this GM B. thuringiensis powder preparation in subacute test was greater than 5000 mg/kg BW. Since both the acute and subacute oral toxicity were not detected at the highest dose recommended by OECD guidelines, this GM B. thuringiensis could be generally regarded as safe for use in bio-pesticide industry.
本研究的目的是评估携带营养期杀虫蛋白（VIP）基因的转基因苏云金芽孢杆菌的毒理学安全性。采用其粉剂制剂对Wistar大鼠进行了急性和亚急性毒性研究。急性研究结果表明，这种转基因苏云金芽孢杆菌粉剂制剂的未观察到有害作用水平（NOAEL）大于5000毫克/千克体重（BW）。在亚急性研究中，对体重增加、食物和水消耗、临床观察、血液学、血清生物化学、器官重量比和组织病理学结果的数据分析表明，对照组和处理组之间没有显著差异。这些结果证明，这种转基因苏云金芽孢杆菌粉剂制剂在亚急性试验中的NOAEL大于5000毫克/千克BW。由于按照经合组织指南推荐的最高剂量未检测到急性和亚急性口服毒性，这种转基因苏云金芽孢杆菌在生物农药行业中一般可被视为安全的。
