Sánchez C, Barrot C, Xifró A, Ortega M, de Aranda I Gómez, Huguet E, Corbella J, Gené M
Legal Medicine Department, Faculty of Medicine, University of Barcelona, Barcelona, Catalonia, Spain.
Forensic Sci Int. 2007 Oct 25;172(2-3):211-7. doi: 10.1016/j.forsciint.2007.01.007. Epub 2007 Feb 22.
Haplotype frequencies for 16 Y-chromosomal short tandem repeat (STR) loci, included in the Y-Filer kit, were determined in 247 unrelated healthy individuals from the Barcelona metropolitan area (Catalonia, NE Spain). After PCR amplification and denaturing PAGE electrophoresis, DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4.1, DYS437, DYS438 and DYS448 loci were typed. The aim of this study is to evaluate the performance in our population of the 16 loci of the Y-chromosome present in the new Y-Filer commercial identification kit, and acquire haplotype frequencies for mathematic processing of the forensic diagnosis in our geographical working area. In this sample, all haplotypes were unique. From the forensic point of view, the combined polymorphisms of the Y-Filer kit provide a high diagnostic efficiency.
我们在来自巴塞罗那大都市区（西班牙东北部加泰罗尼亚）的247名无亲缘关系的健康个体中，测定了Y-Filer试剂盒中包含的16个Y染色体短串联重复序列（STR）位点的单倍型频率。经过PCR扩增和变性聚丙烯酰胺凝胶电泳后，对DYS456、DYS389I、DYS390、DYS389II、DYS458、DYS19、DYS385a/b、DYS393、DYS391、DYS439、DYS635、DYS392、Y GATA H4.1、DYS437、DYS438和DYS448位点进行分型。本研究的目的是评估新型Y-Filer商业鉴定试剂盒中16个Y染色体位点在我们人群中的性能，并获取单倍型频率，以便在我们的地理工作区域对法医诊断进行数学处理。在这个样本中，所有单倍型都是独特的。从法医角度来看，Y-Filer试剂盒的联合多态性提供了很高的诊断效率。
Zotero 插件