Mun Ho-Suk, Oh Eun-Ju, Kim Hyun-Ju, Lee Keun-Hwa, Koh Young-Hwan, Kim Chang-Jin, Hyun Jin-Won, Kim Bum-Joon
Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
Syst Appl Microbiol. 2007 Jul;30(5):401-7. doi: 10.1016/j.syapm.2007.01.003. Epub 2007 Feb 22.
In a previous study, we found that the phylogenetic analysis of partial rpoB sequences can be used effectively to phylogenetically differentiate Streptomyces spp. [B.J. Kim, C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, Y.H. Kook, Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase beta-subunit gene (rpoB) sequences, Int. J. Syst. Evol. Microbiol. 54 (2004) 593-598]. In the present study, we analyzed the partial rpoB gene sequences of 19 reference Streptomyces strains associated with potato scab. Furthermore, to empirically confirm the usefulness of rpoB gene analysis for the phylogenetic differentiation of Streptomyces spp., we applied the proposed system to 27 potato scab isolates obtained from the Korean provinces of Jeju-do and Kangwon-do. Phylogenetic relationships among these isolates using the devised rpoB gene-based methods were generally similar to those reported for 16S rRNA gene-based analysis. Isolates from potato scab lesion in Korea were also clearly differentiated into their phylogenetic groups by this method. In addition, the deduced RpoB amino acid sequences were also found to be useful for differentiating these strains. Our data demonstrate that the rpoB gene-based method can be used as a means of complementing other genetic methods such as 16S rRNA gene analysis or DNA-DNA hybridization to phylogenetically differentiate potato scab related Streptomyces spp.
在之前的一项研究中，我们发现对部分rpoB序列进行系统发育分析可有效地对链霉菌属物种进行系统发育区分[B.J. Kim, C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, Y.H. Kook，基于部分RNA聚合酶β亚基基因（rpoB）序列对链霉菌属和北里孢菌属的系统发育分析，《国际系统与进化微生物学杂志》54 (2004) 593 - 598]。在本研究中，我们分析了19株与马铃薯疮痂病相关的链霉菌参考菌株的部分rpoB基因序列。此外，为了通过实验证实rpoB基因分析对链霉菌属物种系统发育区分的有效性，我们将所提出的系统应用于从韩国济州岛和江原道获得的27株马铃薯疮痂病分离株。使用基于rpoB基因设计的方法对这些分离株进行系统发育关系分析，其结果总体上与基于16S rRNA基因分析所报道的结果相似。通过这种方法，韩国马铃薯疮痂病病变部位的分离株也能清晰地被区分到它们的系统发育组中。此外，推导的RpoB氨基酸序列也被发现可用于区分这些菌株。我们的数据表明，基于rpoB基因的方法可作为一种补充其他遗传方法（如16S rRNA基因分析或DNA - DNA杂交）的手段，用于对与马铃薯疮痂病相关的链霉菌属物种进行系统发育区分。
