Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
FEMS Microbiol Lett. 2010 Apr;305(2):136-42. doi: 10.1111/j.1574-6968.2010.01928.x.
Potato scab is a serious plant disease caused by several Streptomyces sp., and effective control methods remain unavailable. Although antagonistic bacteria and phages against potato scab pathogens have been reported, to the best of our knowledge, there is no information about fungi that are antagonistic to the pathogens. The aim of this study was to isolate fungal antagonists, characterize their phylogenetic positions, determine their antagonistic activities against potato scab pathogens, and highlight their potential use as control agents under lower pH conditions. Fifteen fungal stains isolated from potato field soils were found to have antagonistic activity against three well-known potato scab pathogens: Streptomyces scabiei, Streptomyces acidiscabiei, and Streptomyces turgidiscabiei. These 15 fungal strains were phylogenetically classified into at least six orders and nine genera based on 18S rRNA gene sequencing analysis. These fungal isolates were related to members of the genera Penicillium, Eupenicillium, Chaetomium, Fusarium, Cladosporium, Mortierella, Kionochaeta, Pseudogymnoascus, and Lecythophora. The antagonistic activities of most of the fungal isolates were highly strengthened under the lower pH conditions, suggesting the advantage of combining their use with a traditional method such as soil acidification. This is the first report to demonstrate that phylogenetically diverse fungi show antagonistic activity against major potato scab pathogens. These fungal strains could be used as potential agents to control potato scab disease.
马铃薯疮痂病是一种由几种链霉菌引起的严重植物病害，目前仍缺乏有效的控制方法。虽然已经报道了拮抗菌和噬菌体对马铃薯疮痂病原菌的作用，但据我们所知，还没有关于拮抗菌对抗病原菌的真菌的信息。本研究的目的是分离拮抗菌，对其进行系统发育定位，确定其对三种著名的马铃薯疮痂病原菌的拮抗活性，并强调其在较低 pH 值条件下作为控制剂的潜在用途。从马铃薯田间土壤中分离到的 15 株真菌菌株被发现对三种著名的马铃薯疮痂病原菌（疮痂链霉菌、酸性链霉菌和肿胀链霉菌）具有拮抗活性。基于 18S rRNA 基因测序分析，这 15 株真菌菌株被系统发育分类为至少六个目和九个属。这些真菌分离物与青霉属、小丛壳属、毛壳属、镰刀菌属、枝孢属、茎点霉属、尾孢属、拟青霉属、假丝酵母属和小皮伞属的成员有关。大多数真菌分离物在较低 pH 条件下的拮抗活性得到了高度增强，这表明将其与土壤酸化等传统方法结合使用具有优势。这是首次报道表明，系统发育多样的真菌对主要的马铃薯疮痂病原菌具有拮抗活性。这些真菌菌株可以用作控制马铃薯疮痂病的潜在药剂。
