Bloom Kerry
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Curr Opin Genet Dev. 2007 Apr;17(2):151-6. doi: 10.1016/j.gde.2007.02.009. Epub 2007 Feb 22.
At the foundation of all eukaryotic kinetochores is a unique histone variant, known as CenH3 (centromere histone H3). We are starting to identify the histone chaperones responsible for CenH3 deposition at centromere DNA, and the mechanisms that restrict CenH3 from chromosome arms. The specialized nucleosome that contains CenH3 in place of canonical histone H3 lies at the interface between microtubules and chromosomes and directs kinetochore protein assembly. By contrast, pericentric chromatin is highly elastic and can stretch or recoil in response to microtubule shortening or growth in mitosis. The variety in histone modification is likely to play a key role in regulating the behavior of these distinct chromatin domains.
所有真核生物动粒的基础是一种独特的组蛋白变体，称为着丝粒组蛋白H3（CenH3）。我们开始鉴定负责将CenH3沉积在着丝粒DNA上的组蛋白伴侣，以及将CenH3限制在染色体臂之外的机制。含有CenH3而非经典组蛋白H3的特殊核小体位于微管和染色体之间的界面，并指导动粒蛋白组装。相比之下，着丝粒周围染色质具有高度弹性，可在有丝分裂中响应微管缩短或生长而伸展或回缩。组蛋白修饰的多样性可能在调节这些不同染色质结构域的行为中起关键作用。
