肾细胞癌患者肿瘤组织及尿液中脑型脂肪酸结合蛋白转录本的检测

Detection of transcript for brain-type fatty Acid-binding protein in tumor and urine of patients with renal cell carcinoma.

作者信息

Teratani Takumi, Domoto Tomohiro, Kuriki Ken, Kageyama Teruyo, Takayama Tatsuya, Ishikawa Akira, Ozono Seiichiro, Nozawa Ryushi

机构信息

Laboratory of Host Defenses, University of Shizuoka Graduate School of Health Science, Suruga-ku, Shizuoka, Japan.

出版信息

Urology. 2007 Feb;69(2):236-40. doi: 10.1016/j.urology.2006.09.060.

DOI:10.1016/j.urology.2006.09.060

PMID:17320655

Abstract

OBJECTIVES

To clarify the gene expression patterns of fatty acid-binding protein (FABP) and evaluate it as a potential marker for the diagnosis of renal cell carcinoma (RCC). RCC is the most common renal neoplasm.

METHODS

The expression of eight FABP genes in normal human tissues, tumor cell lines, and surgically resected RCC tissues (n = 54) was evaluated by reverse transcriptase-polymerase chain reaction. Additionally, the gene expression of FABPs in the urine of healthy volunteers (n = 12) and patients with RCC (n = 5) was investigated.

RESULTS

In these results, the carcinoma tissues but not the noncancerous (normal) parts of the kidney samples resected from patients with RCC expressed the transcript for brain-type FABP (B-FABP), indicating that expression of the B-FABP gene is a novel marker for RCC. Furthermore, the B-FABP cDNA fragment was not amplified by reverse transcriptase-polymerase chain reaction in the urine samples of healthy donors or patients with RCC after surgical operation. However, B-FABP cDNA was amplified in the patients' urine samples collected before surgery.

CONCLUSIONS

This novel method can be used as a powerful ancillary in the diagnosis of RCC.

摘要

目的

阐明脂肪酸结合蛋白（FABP）的基因表达模式，并评估其作为肾细胞癌（RCC）诊断潜在标志物的价值。RCC是最常见的肾脏肿瘤。

方法

通过逆转录-聚合酶链反应评估8种FABP基因在正常人体组织、肿瘤细胞系及手术切除的RCC组织（n = 54）中的表达。此外，还研究了健康志愿者（n = 12）和RCC患者（n = 5）尿液中FABP的基因表达。

结果

在这些结果中，从RCC患者切除的肾脏样本的癌组织而非非癌（正常）部分表达脑型FABP（B-FABP）的转录本，表明B-FABP基因的表达是RCC的一种新标志物。此外，健康供体或手术后RCC患者的尿液样本经逆转录-聚合酶链反应未扩增出B-FABP cDNA片段。然而，术前收集的患者尿液样本中扩增出了B-FABP cDNA。