大肠杆菌硫醇刺激卟啉分泌的酶学基础。
Enzymatic basis of thiol-stimulated secretion of porphyrins by Escherichia coli.
作者信息
Javor G T, Febre E F
机构信息
Department of Biochemistry and Microbiology, Loma Linda University School of Medicine, California 92350.
出版信息
J Bacteriol. 1992 Feb;174(3):1072-5. doi: 10.1128/jb.174.3.1072-1075.1992.
Abstract
1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined. TG treatment elevated the activity of glutamyl-tRNA reductase in both strains. The increased activity was not caused by activation of preexisting enzymes by thiols or by oxidizing agents but was dependent on new protein synthesis.
摘要
1-硫代甘油（TG）刺激需氧生长的大肠杆菌中卟啉的合成。在此测定了未处理和经TG处理的大肠杆菌THU和PUC2（THU的一个突变体，在硫醇存在下过量产生卟啉）细胞中δ-氨基乙酰丙酸生物合成酶的水平。TG处理提高了两种菌株中谷氨酰-tRNA还原酶的活性。活性增加并非由硫醇或氧化剂对现有酶的激活所致，而是依赖于新的蛋白质合成。
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本文引用的文献
1
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Plant Physiol. 1988 Nov;88(3):879-86. doi: 10.1104/pp.88.3.879.
2
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
3
THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS. I. STUDIES WITH THYMINELESS STRAINS OF ESCHERICHIA COLI.无蛋白质合成情况下信使核糖核酸的合成。I. 对大肠杆菌无胸腺嘧啶菌株的研究。
J Mol Biol. 1964 May;8:629-37. doi: 10.1016/s0022-2836(64)80113-3.
4
delta-aminolaevulate dehydratase from Rhodopseudomonas spheroides.来自球形红假单胞菌的δ-氨基乙酰丙酸脱水酶
Biochem J. 1963 Jun;87(3):462-72. doi: 10.1042/bj0870462.
5
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