Warpeha Katherine M, Upadhyay Snehali, Yeh Jennifer, Adamiak Julia, Hawkins Samuel I, Lapik Yevgeniya R, Anderson Mary Beth, Kaufman Lon S
Laboratory for Molecular Biology, Department of Biological Sciences , University of Illinois, Chicago, Illinois 60607, USA.
Plant Physiol. 2007 Apr;143(4):1590-600. doi: 10.1104/pp.106.089904. Epub 2007 Feb 23.
Different classes of biotic (e.g. plant hormones) and abiotic (e.g. different wavelengths of light) signals act through specific signal transduction mechanisms to coordinate higher plant development. While a great deal of progress has been made, full signal transduction chains have not yet been described for most blue light- or abscisic acid-mediated events. Based on data derived from T-DNA insertion mutants and yeast (Saccharomyces cerevisiae) two-hybrid and coprecipitation assays, we report a signal transduction chain shared by blue light and abscisic acid leading to light-harvesting chlorophyll a/b-binding protein expression in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. The chain consists of GCR1 (the sole Arabidopsis protein coding for a potential G-protein-coupled receptor), GPA1 (the sole Arabidopsis Galpha-subunit), Pirin1 (PRN1; one of four members of an iron-containing subgroup of the cupin superfamily), and a nuclear factor Y heterotrimer comprised of A5, B9, and possibly C9. We also demonstrate that this mechanism is present in imbibed seeds wherein it affects germination rate.
不同类别的生物信号（如植物激素）和非生物信号（如不同波长的光）通过特定的信号转导机制发挥作用，以协调高等植物的发育。尽管已经取得了很大进展，但对于大多数蓝光或脱落酸介导的事件，完整的信号转导链尚未被描述。基于来自T-DNA插入突变体以及酵母（酿酒酵母）双杂交和共沉淀实验的数据，我们报道了一条蓝光和脱落酸共有的信号转导链，该转导链导致黄化拟南芥幼苗中光捕获叶绿素a/b结合蛋白的表达。该转导链由GCR1（拟南芥中唯一编码潜在G蛋白偶联受体的蛋白）、GPA1（拟南芥中唯一的Gα亚基）、Pirin1（PRN1；豆球蛋白超家族含铁亚组的四个成员之一）以及由A5、B9和可能的C9组成的核因子Y异源三聚体构成。我们还证明，这种机制存在于吸胀种子中，它会影响种子的萌发率。
