Genome-Wide Identification and Drought Stress Response Pattern of the NF-Y Gene Family in .

a type of orchid plant, is more drought-resistant and ornamental than other terrestrial orchids. Research has shown that many members of the transcription factor family are responsive to plant growth, development, and abiotic stress. However, the mechanism of the gene family's response to abiotic stress in orchids has not yet been reported. In this study, phylogenetic analysis allowed for 27 genes to be identified (5 , 9 , and 13 subunits), and the s were homologous to those in and . Protein structure analysis revealed that different subfamilies contained different motifs, but all of them contained Motif 2. Secondary and tertiary protein structure analysis indicated that the and subfamilies had a high content of alpha helix structures. -element analysis showed that elements related to drought stress were mainly concentrated in the and subfamilies, with and having the highest content. The results of a transcriptome analysis showed that there was a trend of downregulation of almost all in leaves under drought stress, while in roots, most members of the subfamily showed a trend of upregulation. Additionally, seven genes were selected for real-time reverse transcription quantitative PCR (qRT-PCR) experiments. The results were generally consistent with those of the transcriptome analysis. The regulatory roles of , , and were particularly evident in the roots. The findings of our study may make a great contribution to the understanding of the role of in stress-related metabolic processes.