乙酰胆碱受体抗体复合物在实验性自身免疫性重症肌无力肌肉中的作用
Role of acetylcholine receptor antibody complexes in muscle in experimental autoimmune myasthenia gravis.
作者信息
Verschuuren J J, Graus Y M, Theunissen R O, Yamamoto T, Vincent A, van Breda Vriesman P J, De Baets M H
机构信息
Department of Immunology, University of Limburg, Maastricht, Netherlands.
出版信息
J Neuroimmunol. 1992 Feb;36(2-3):117-25. doi: 10.1016/0165-5728(92)90043-k.
In experimental autoimmune myasthenia gravis anti-rat nicotinic acetylcholine receptor (AChR) antibody titers correlated significantly with the AChR-antibody complexes found in muscle. It was shown that at least a large part of the AChR-antibody complexes are formed in vitro, which can be prevented by washing of the muscle homogenate. Using a modified assay, no differences in AChR-antibody complexes could be detected between rats with and without symptoms of experimental autoimmune myasthenia gravis. Also no difference in AChR loss nor in inhibition of alpha-bungarotoxin binding to AChR was found between these groups of rats. However, a significant difference in the reduction of AChR function was found, using an assay measuring agonist-induced 22Na+ flux into the TE671 cell line.
在实验性自身免疫性重症肌无力中，抗大鼠烟碱型乙酰胆碱受体（AChR）抗体滴度与肌肉中发现的AChR抗体复合物显著相关。结果表明，至少大部分AChR抗体复合物是在体外形成的，这可以通过洗涤肌肉匀浆来预防。使用改良的检测方法，在有和没有实验性自身免疫性重症肌无力症状的大鼠之间未检测到AChR抗体复合物的差异。在这些大鼠组之间，AChR丢失以及α-银环蛇毒素与AChR结合的抑制也没有差异。然而，使用测量激动剂诱导的22Na+流入TE671细胞系的检测方法，发现AChR功能降低存在显著差异。
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