Pinto Magali Luci, Tokunaga Heloisa Helena Vieira Olyntho, Souccar Caden, Schoorlemmer Guus H M, Lapa Rita de Cássia Ribeiro da Silva
Department of Histology, Universidade Federal de São Paulo, São Paulo, Brazil.
Neurosci Lett. 2007 Apr 12;416(2):175-9. doi: 10.1016/j.neulet.2007.02.004. Epub 2007 Feb 7.
The lack of dystrophin that causes Duchenne muscle disease affects not only the muscles but also the central nervous system. Dystrophin-deficient mdx mice present changes in several brain fiber systems. We compared the projections from the trigeminal sensory nuclear complex to the red nucleus in control and mdx mice using retrograde tracers. Injection of 200 nL 2% fluorogold into the red nucleus caused labeling in the mesencephalic trigeminal nucleus, the principal sensory nucleus and the oral, interpolar, and caudal subnuclei of the spinal trigeminal nucleus in both control and mdx mice. Injection of latex microbeads labeled with rhodamine and fluorescein gave results similar to those seen with fluorogold. The number of labeled neurons in the trigeminal sensory nuclear complex was significantly reduced in mdx mice. In the oral subnucleus of the spinal trigeminal nucleus this reduction was 50%. These results indicate that the trigemino-rubral pathway is reduced in dystrophin-deficient mice.
导致杜兴氏肌肉病的肌营养不良蛋白缺失不仅会影响肌肉，还会影响中枢神经系统。缺乏肌营养不良蛋白的mdx小鼠在多个脑纤维系统中出现变化。我们使用逆行示踪剂比较了对照小鼠和mdx小鼠中从三叉神经感觉核复合体到红核的投射。向红核注射200 nL 2%的荧光金导致在对照小鼠和mdx小鼠的中脑三叉神经核、主感觉核以及脊髓三叉神经核的口侧、极间和尾侧亚核中出现标记。注射用罗丹明和荧光素标记的乳胶微珠得到的结果与荧光金相似。mdx小鼠三叉神经感觉核复合体中标记神经元的数量显著减少。在脊髓三叉神经核的口侧亚核中，这种减少为50%。这些结果表明，在缺乏肌营养不良蛋白的小鼠中，三叉神经-红核通路减少。
