Oh Joo Youn, In Youn Seok, Kim Mee Kum, Ko Jung Hwa, Lee Hyun Ju, Shin Ki Cheul, Lee Sang Mok, Wee Won Ryang, Lee Jin Hak, Park Myunggyu
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.
Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1102-9. doi: 10.1167/iovs.06-0809.
To investigate the effect of uridine on cultured human corneal epithelial cells and keratocytes in vitro and to evaluate whether the application of uridine-containing eye drops could improve ocular surface health in an in vivo dry eye model.
Uridine was added to cultured epithelial cells (3 x 10(4) cells/well) and keratocytes (1 x 10(4) cells/well) at various concentrations (0.5-50 microM). Cytotoxicity was tested with the use of MTT assay, and the cells were assessed for apoptosis with the use of flow cytometry. Expressions of hyaluronic acid (HA), glycosaminoglycan (GAG), nitric oxide (NO), and matrix metalloproteinase (MMP)-9 were measured. In vivo, the degree of reepithelialization was assessed after topical application of uridine (100 microM) in a rabbit corneal wound model. Changes in tear production and conjunctival goblet cell counts were investigated after instillation of various concentrations of uridine-containing eye drops in a rabbit dry eye model.
In vitro, uridine showed no cellular toxicity. It increased the biosynthesis of HA and GAG and reduced MMP-9 levels in cultured corneal epithelial cells and keratocytes. In vivo, uridine enhanced corneal wound healing and significantly increased the number of conjunctival goblet cells in rabbits.
Uridine can restore the health of the ocular surface in a rabbit corneal wound and dry eye model.
研究尿苷对体外培养的人角膜上皮细胞和角膜细胞的影响，并评估含尿苷滴眼液的应用是否能改善体内干眼模型的眼表健康。
将不同浓度（0.5 - 50微摩尔）的尿苷添加到培养的上皮细胞（3×10⁴个细胞/孔）和角膜细胞（1×10⁴个细胞/孔）中。使用MTT法检测细胞毒性，并使用流式细胞术评估细胞凋亡情况。检测透明质酸（HA）、糖胺聚糖（GAG）、一氧化氮（NO）和基质金属蛋白酶（MMP）-9的表达。在体内，在兔角膜伤口模型中局部应用尿苷（100微摩尔）后评估再上皮化程度。在兔干眼模型中滴入不同浓度的含尿苷滴眼液后，研究泪液分泌和结膜杯状细胞计数的变化。
在体外，尿苷无细胞毒性。它增加了培养的角膜上皮细胞和角膜细胞中HA和GAG的生物合成，并降低了MMP-9水平。在体内，尿苷促进了兔角膜伤口愈合，并显著增加了结膜杯状细胞的数量。
尿苷可恢复兔角膜伤口和干眼模型中的眼表健康。
