Han Yan, Zhou Ya, Liu Quan
Dept. of Microbiology and Immunology, Ningxia Medical College, Yinchuan 750004, China.
Zhong Yao Cai. 2006 Oct;29(10):1066-9.
To investigate the effect and explore the mechanism of protective role exerted by Total Alkaloids of Sophora Alopecuroides and three kinds of pharmacoalkaloids (Sophocarpine, Sophoridine, Oxymatrine) in mouse model with acute lung injury.
Qingkailing Injection was used as positive control. The mouse model with lung injury induced endotoxin was established by peritoneal injection of LPS 1 h after the last administration. The ICR mice were pretreated with Total Alkaloids of Sophora Alopecuroides 25 mg/kg, Sophoridine 5 mg/kg, Oxymatrine 50 mg/kg and Sophocarpine 25 mg/kg for 3 days respectively. The anti-endotoxin effect of Total Alkaloids of Sophora Alopecuroides and the three kinds of pharmacoalkoids in vivo was studied by the general symptoms, white blood cell count, lung wet/dry weight, pathological histologic changes of lung tissues. CD14 and SR-A of lung tissue and serum TNF-alpha and IL-6 were detected by immunohistochemical and radioimmunity method.
The general symptoms were improved, the number of white blood cells were increased, lung wet/dry weight was decreased and pathological changes of lung tissues were milder at different level. Meanwhile the expression of CD14 was decreased but the expression of SR-A increased in the lung tissue. Both the level of serum TNF-alpha and IL-6 were decrased.
The damage of lung tissue in the mice with endotoxemia is inhibited by the Total Alkalids of Sophora Alopecuroides and the three kinds of pharmacoalkaloids at different level. The anti-endotoxin mechanism is associated with the regulating expression of LPS recognition receptor and down-regulating the expression of the down-stream inflammatory factors.
研究苦豆子总生物碱及三种生物碱（槐果碱、槐定碱、氧化苦参碱）对急性肺损伤小鼠模型的保护作用及机制。
以清开灵注射液为阳性对照。末次给药1 h后腹腔注射脂多糖建立内毒素诱导的小鼠肺损伤模型。将ICR小鼠分别用苦豆子总生物碱25 mg/kg、槐定碱5 mg/kg、氧化苦参碱50 mg/kg和槐果碱25 mg/kg预处理3天。通过观察一般症状、白细胞计数、肺湿/干重、肺组织病理组织学变化，研究苦豆子总生物碱及三种生物碱在体内的抗内毒素作用。采用免疫组织化学和放射免疫法检测肺组织CD14、SR-A及血清TNF-α、IL-6。
不同程度改善了一般症状，增加了白细胞数量，降低了肺湿/干重，减轻了肺组织病理变化。同时肺组织中CD14表达降低，SR-A表达增加。血清TNF-α、IL-6水平均降低。
苦豆子总生物碱及三种生物碱不同程度抑制了内毒素血症小鼠肺组织损伤。抗内毒素机制与调节LPS识别受体表达及下调下游炎症因子表达有关。
