Xing Wei, Zuo Jian-E, Sun Yu-Jiao, Li Jian-Ping
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Huan Jing Ke Xue. 2006 Nov;27(11):2268-72.
Four methanogenic granules taken from an anaerobic reactor in different periods were investigated by FISH and DGGE, the eubacterial and archaeal community in these granules was researched and the phylogenetic analysis of dominant archaea was also studied. The FISH results indicated that the quantity of eubacteria was much more than archaea in the methanogenic granule and most eubacteria were located in the out layer of granule, while most archaea were located in the inner layer. The DGGE fingerprints indicated that as the organic loading rate of the reactor increased and the operating time elapsed, the eubacterial community was kept stable relatively, while the archaeal community was changed significantly, which resulted in the gradual decrease of the archaeal varieties. As seven typical bands were cut and sequenced, the results indicated that the dominant species of archaea in granule of the last period were Methanocor pusculum, Methanobacterium, Methanosaeta, and etc.
对取自厌氧反应器不同时期的4个产甲烷颗粒进行了荧光原位杂交（FISH）和变性梯度凝胶电泳（DGGE）分析，研究了这些颗粒中的真细菌和古细菌群落，并对优势古菌进行了系统发育分析。FISH结果表明，产甲烷颗粒中真细菌的数量远多于古细菌，且大多数真细菌位于颗粒外层，而大多数古细菌位于颗粒内层。DGGE指纹图谱表明，随着反应器有机负荷率的增加和运行时间的推移，真细菌群落相对保持稳定，而古细菌群落发生了显著变化，导致古细菌种类逐渐减少。对7条典型条带进行切割测序后，结果表明，最后一个时期颗粒中古菌的优势种为微小甲烷球菌、甲烷杆菌、甲烷丝状菌等。
