Sun Y J, Xing W, Li J P, Lu Y Q, Zuo J E
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Prikl Biokhim Mikrobiol. 2009 Nov-Dec;45(6):659-63.
The spatial distribution, quantity and diversity of different microorganisms within anaerobic granular sludge from a lab-scale expanded granular sludge bed (EGSB) reactor operated at different organic loading rates were studied using florescent in situ hybridization (FISH), real time quantitative-polymerase chain reaction (RTQ-PCR) and denaturing gradient gel electrophoresis (DGGE) techniques. The results indicated that most Eubacteria were located in the outer layer of granule, while the Archaea which mainly were methanogens and more sensible to the environmental conditions were located in the inner layer of the granule. The quantity of Archaea was obviously less than that of Eubacteria in the granules, but increased with the increasing of organic loading rates of the reactor. As the organic loading rate of the reactor increased and the operating time elapsed, the Archaea community in the granules changed significantly. Seven typical DGGE bands were collected and sequenced, and found that the dominant species of Archaea in the granules operated in the last period were mainly Methanocorpusculum, Methanobacterium, Methanosaeta.
利用荧光原位杂交（FISH）、实时定量聚合酶链反应（RTQ-PCR）和变性梯度凝胶电泳（DGGE）技术，研究了在不同有机负荷率下运行的实验室规模膨胀颗粒污泥床（EGSB）反应器中厌氧颗粒污泥内不同微生物的空间分布、数量和多样性。结果表明，大多数真细菌位于颗粒外层，而主要为产甲烷菌且对环境条件更敏感的古菌位于颗粒内层。颗粒中古菌数量明显少于真细菌，但随着反应器有机负荷率的增加而增加。随着反应器有机负荷率的提高和运行时间的推移，颗粒中的古菌群落发生了显著变化。收集并测序了7条典型的DGGE条带，发现最后阶段运行的颗粒中古菌的优势种主要为小甲烷八叠球菌、甲烷杆菌、甲烷丝状菌。
