Gross-Hardt Rita, Kägi Christina, Baumann Nadine, Moore James M, Baskar Ramamurthy, Gagliano Wendy B, Jürgens Gerd, Grossniklaus Ueli
Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland.
PLoS Biol. 2007 Mar;5(3):e47. doi: 10.1371/journal.pbio.0050047.
In flowering plants, the egg and sperm cells form within haploid gametophytes. The female gametophyte of Arabidopsis consists of two gametic cells, the egg cell and the central cell, which are flanked by five accessory cells. Both gametic and accessory cells are vital for fertilization; however, the mechanisms that underlie the formation of accessory versus gametic cell fate are unknown. In a screen for regulators of egg cell fate, we isolated the lachesis (lis) mutant which forms supernumerary egg cells. In lis mutants, accessory cells differentiate gametic cell fate, indicating that LIS is involved in a mechanism that prevents accessory cells from adopting gametic cell fate. The temporal and spatial pattern of LIS expression suggests that this mechanism is generated in gametic cells. LIS is homologous to the yeast splicing factor PRP4, indicating that components of the splice apparatus participate in cell fate decisions.
在开花植物中，卵细胞和精细胞在单倍体配子体中形成。拟南芥的雌配子体由两个配子细胞，即卵细胞和中央细胞组成，它们两侧是五个辅助细胞。配子细胞和辅助细胞对于受精都至关重要；然而，辅助细胞与配子细胞命运形成的潜在机制尚不清楚。在一项对卵细胞命运调节因子的筛选中，我们分离出了形成额外卵细胞的lachesis（lis）突变体。在lis突变体中，辅助细胞分化为配子细胞命运，这表明LIS参与了一种防止辅助细胞采用配子细胞命运的机制。LIS表达的时空模式表明这种机制是在配子细胞中产生的。LIS与酵母剪接因子PRP4同源，这表明剪接装置的成分参与了细胞命运的决定。
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