Skinner Debra J, Dang Trang, Gasser Charles S
Dept. of Molecular and Cellular Biology University of California-Davis Davis California USA.
Present address: Dept. of Plant Biology University of California-Davis Davis California USA.
Plant Direct. 2023 Feb 22;7(2):e485. doi: 10.1002/pld3.485. eCollection 2023 Feb.
The () gene is essential for formation of the outer integument of ovules in . Initially described lesions in were missense mutations resulting in aberrant mRNA splicing. To determine the null mutant phenotype, we generated frameshift mutations and found, in confirmation of results on another recently identified frameshift mutation, that such mutants have a phenotype identical to the most severe splicing mutant (), with effects specific to outer integument development. We show that the altered protein of an mRNA splicing mutant with a less severe phenotype () does not have activity, and the mutant is partial because it produces a small amount of correctly spliced mRNA. Screening for suppressors of in a fast neutron-mutagenized population identified a translocated duplication of the gene, leading to an increase in the amount of this mRNA. The increased expression led to a decrease in the severity of the mutant effects, indicating that the amount of activity quantitatively regulates outer integument growth. The results further confirm that the role of in Arabidopsis development is specific to the outer integument of ovules where it quantitatively affects the growth of this structure.
（）基因对于[植物名称]胚珠外珠被的形成至关重要。最初在[植物名称]中描述的损伤是错义突变，导致异常的mRNA剪接。为了确定无效突变体表型，我们产生了移码突变，并在证实另一个最近鉴定的移码突变结果时发现，此类突变体具有与最严重的剪接突变体（）相同的表型，对外珠被发育具有特异性影响。我们表明，具有较轻表型（）的mRNA剪接突变体的改变蛋白不具有[蛋白功能]活性，并且该突变体是部分性的，因为它产生少量正确剪接的mRNA。在快中子诱变群体中筛选[突变体名称]的抑制子时，鉴定出该基因的易位重复，导致该mRNA量增加。表达增加导致突变效应的严重程度降低，表明[蛋白功能]活性的量定量调节外珠被生长。结果进一步证实，[基因名称]在拟南芥发育中的作用对外珠被是特异性的，在那里它定量地影响该结构的生长。
