Haussmann Mark F, Vleck Carol M, Farrar Eugenia S
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA.
Adv Physiol Educ. 2007 Mar;31(1):110-5. doi: 10.1152/advan.00058.2006.
Perceived stress activates the hypothalamus-pituitary-adrenal axis, resulting in the release of glucocorticoids into the systemic circulation. Glucocorticoids cause the elevation of blood glucose, providing the necessary energy for the organism to cope with stress. Here, we outline a laboratory exercise that uses a competitive ELISA kit to illustrate the response of salivary cortisol concentrations to three stressful conditions. Twelve undergraduate students in the General and Comparative Endocrinology course at Iowa State University were subjected to presentation stress, fasting stress, and competition stress to determine their effect on salivary cortisol concentrations. Students had elevated salivary cortisol in response to each of these stresses compared with basal conditions. These results reiterate the importance of glucocorticoids as mediators of the stress response. This study also incorporates the use of the ELISA technique, a modern laboratory tool used to determine the amount of endogenous antigens in plasma or saliva. This laboratory exercise can easily be adapted to fit into already existing physiology and endocrinology curriculums.
感知到的压力会激活下丘脑-垂体-肾上腺轴，导致糖皮质激素释放到体循环中。糖皮质激素会使血糖升高，为机体应对压力提供必要的能量。在此，我们概述一项实验室实验，该实验使用竞争性酶联免疫吸附测定试剂盒来说明唾液皮质醇浓度对三种应激条件的反应。爱荷华州立大学普通与比较内分泌学课程的12名本科生经历了演讲压力、禁食压力和竞争压力，以确定这些压力对唾液皮质醇浓度的影响。与基础状态相比，学生们在应对这些压力时唾液皮质醇水平均有所升高。这些结果重申了糖皮质激素作为应激反应介质的重要性。本研究还采用了酶联免疫吸附测定技术，这是一种用于测定血浆或唾液中内源性抗原量的现代实验室工具。这个实验室实验可以很容易地进行调整，以适应现有的生理学和内分泌学课程。
