Bentancor A, Rumi M V, Gentilini M V, Sardoy C, Irino K, Agostini A, Cataldi A
Area Microbiología, facultad de Ciencias veterinarias, Universidad de Buenos Aires, Chorroarín 280, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
FEMS Microbiol Lett. 2007 Feb;267(2):251-6. doi: 10.1111/j.1574-6968.2006.00569.x.
Shiga toxin-producing Escherichia coli (STEC), responsible for the hemolytic uremic syndrome, is an endemic pathogen in Argentina. We studied the prevalence of STEC in fecal samples from cats and dogs of Buenos Aires city and suburbs. Cultures were used for screening stx1/stx2 and rfbO157 by multiplex PCR. All E. coli-positive colonies for these genes were further characterized for the eae gene and for serotypes. In dogs, 17 (3.7%), 19 (4.2%) and 34 (7.5%) of samples were positive for stx2, stx1 and rfb, respectively. In cats, six (4.0%) of the samples were positive for stx2, three (2.0%) for stx1 and four (2.7%) for rfbO157. In 18 (4.0%) of the dog samples, a bacteriological diagnosis was obtained by isolation. The percentage of positive isolates corresponding to the rfbO157 and to the stx2 genotypes were 2.9% and 1.1%, respectively. In four of the cat samples, the bacteriological diagnosis for stx2 (2.6% prevalence of STEC) was confirmed. Although these data suggest that the high infection index of STEC in children in Argentina does not seem to be due mainly to the role of cats and dogs, there are some strains with virulence genes in common for humans and their domestic animals.
产志贺毒素大肠杆菌（STEC）是导致溶血性尿毒症综合征的病原体，在阿根廷属于地方性流行病菌。我们研究了布宜诺斯艾利斯市及周边地区猫和狗粪便样本中STEC的流行情况。通过多重聚合酶链反应（PCR），利用培养法筛选stx1/stx2和rfbO157。对所有这些基因呈阳性的大肠杆菌菌落进一步进行eae基因和血清型鉴定。在狗的样本中，stx2、stx1和rfb分别有17份（3.7%）、19份（4.2%）和34份（7.5%）呈阳性。在猫的样本中，stx2有6份（4.0%）呈阳性，stx1有3份（2.0%）呈阳性，rfbO157有4份（2.7%）呈阳性。在18份（4.0%）狗的样本中，通过分离获得了细菌学诊断结果。对应rfbO157和stx2基因型的阳性分离株百分比分别为2.9%和1.1%。在4份猫的样本中确诊了stx2的细菌学诊断结果（STEC患病率为2.6%）。尽管这些数据表明阿根廷儿童中STEC的高感染指数似乎并非主要归因于猫和狗，但人类及其家畜中存在一些具有共同毒力基因的菌株。
