Thomadaki Hellinida, Karaliota Alexandra, Litos Charalambos, Scorilas Andreas
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, 15701 Athens, Greece.
J Med Chem. 2007 Mar 22;50(6):1316-21. doi: 10.1021/jm0610797. Epub 2007 Mar 1.
We synthesized and studied the antitumor properties of the novel complex compound 2,5-dihydroxybenzoate molybdenum(VI) with tetraphenylphosphonium as counterion, which also acts as cancer cell growth inhibitor. A novel complex compound, the 2,5-dihydroxybenzoate molybdenum(VI) complex, (PPh4)2[Mo3O6(mu-O)2(2,5-DHBA)2] was synthesized. 1H NMR, 13C NMR, IR, and UV-Vis analyses were used for its molecular characterization. The human leukemia cell lines HL-60 and K562 were tested for their viability by assessing the metabolic activity of cells (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MTT), the structural integrity of the cell membrane (Trypan blue assay), and cell proliferation ability (growth curves). We showed that both leukemia cell lines are induced to decreased proliferation efficiency after treatment with the novel complex, compared to 2,5-dihydroxybenzoate, tetraphenyl-phosphonium polyoxomolybdate, or tetraphenylphosphonium chloride as individual entities, in a time- and concentration-dependent manner. Our results suggest that the new 2,5-dihydroxybenzoate molybdenum(VI) complex may provide a valuable tool in cancer chemotherapy.
我们合成并研究了以四苯基鏻为抗衡离子的新型配合物2,5 - 二羟基苯甲酸钼(VI)的抗肿瘤特性，该配合物也可作为癌细胞生长抑制剂。合成了一种新型配合物，即2,5 - 二羟基苯甲酸钼(VI)配合物(PPh4)2[Mo3O6(μ - O)2(2,5 - DHBA)2]。利用1H NMR、13C NMR、红外光谱和紫外 - 可见光谱分析对其进行分子表征。通过评估细胞的代谢活性（3-(4,5 - 二甲基噻唑 - 2 - 基)-2,5 - 二苯基四氮唑溴盐；MTT）、细胞膜的结构完整性（台盼蓝测定法）和细胞增殖能力（生长曲线），检测了人白血病细胞系HL - 60和K562的活力。我们发现，与单独使用2,5 - 二羟基苯甲酸、多氧钼酸四苯基鏻盐或四苯基氯化鏻相比，用该新型配合物处理后，两种白血病细胞系的增殖效率均呈时间和浓度依赖性降低。我们的结果表明，新型2,5 - 二羟基苯甲酸钼(VI)配合物可能为癌症化疗提供一种有价值的工具。
