Jóźwiak Jarosław, Grzela Tomasz, Jankowska-Steifer Ewa, Komar Aldona, Moskalewski Stanisław, Martirosian Gayane
Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
FEMS Immunol Med Microbiol. 2007 Mar;49(2):296-303. doi: 10.1111/j.1574-695X.2006.00204.x.
In this study, for the first time, Clostridium histolyticum lethal factor was purified by ammonium sulfate precipitation of culture broth, centrifugation through an Amicon filter device and hydrophobic interaction chromatography. The purified lethal factor was devoid of proteolytic activity. At a concentration of 150 ng mL(-1) the lethal factor killed 50% of HeLa cells within 24 h of exposure. Abrogation of actin filaments, activation of caspases, fragmentation of nuclear DNA as well as ultrastructural changes indicated that the cell death occurred by apoptosis. The apoptotic action of the lethal factor is in agreement with changes induced in animal tissues by administration of C. histolyticum culture medium.
在本研究中，首次通过对培养液进行硫酸铵沉淀、使用Amicon过滤装置离心以及疏水相互作用色谱法来纯化溶组织梭菌致死因子。纯化后的致死因子没有蛋白水解活性。在浓度为150 ng mL(-1)时，致死因子在暴露24小时内可杀死50%的HeLa细胞。肌动蛋白丝的消除、半胱天冬酶的激活、核DNA的片段化以及超微结构的变化表明细胞死亡是由凋亡引起的。致死因子的凋亡作用与给动物组织注射溶组织梭菌培养基所诱导的变化一致。
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