聚合物酶电极检测农药

Detection of pesticide by polymeric enzyme electrodes.

作者信息

Dutta K, Bhattacharyay D, Mukherjee A, Setford S J, Turner A P F, Sarkar P

机构信息

Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, Kolkata 700009, India.

出版信息

Ecotoxicol Environ Saf. 2008 Mar;69(3):556-61. doi: 10.1016/j.ecoenv.2007.01.004. Epub 2007 Feb 27.

DOI:10.1016/j.ecoenv.2007.01.004

PMID:17328951

Abstract

Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (microg L(-1)).

摘要

含有固定化乙酰胆碱酯酶（AChE）的丝网印刷电极（SPEs）用于有机磷（OP）和氨基甲酸酯类农药的电化学测定。在乙酰胆碱（AChCl）底物存在的情况下，测定农药对AChE的失活程度。这种方法的独特之处在于酶固定化过程，其中通过丙烯酰胺的原位本体聚合将AChE附着到SPE上，以确保在膜内有效附着，同时酶活性损失最小。在0-10 ppb（微克/升）的浓度范围内，观察到了久效磷、马拉硫磷、氧化乐果和杀螟丹等农药的响应。