Arizza Vincenzo, Giaramita Francesca Tiziana, Parrinello Daniela, Cammarata Matteo, Parrinello Nicolò
Department of Animal Biology, University of Palermo, Palermo, Italy.
Comp Biochem Physiol A Mol Integr Physiol. 2007 Jun;147(2):389-94. doi: 10.1016/j.cbpa.2007.01.022. Epub 2007 Feb 1.
The coelomic fluid from the sea urchin Paracentrotus lividus contains several coelomocyte types including amoebocytes and uncoloured spherulocytes involved in immune defences. In the present paper, we show a Ca(2+)-dependent cytotoxic activity for the unfractionated coelomocytes assayed in vitro, with rabbit erythrocytes and the K562 tumour cell line. In a plaque-forming assay, whole coelomocyte preparations as well as density gradient separated coelomocyte populations revealed that cell populations enriched in uncoloured spherulocytes, exerted high cytotoxic activity by releasing lysins in the presence of amoebocytes. This cooperative effect could be dependent on soluble factors released by amoebocytes. With regard to this, we show that an enhanced cytotoxic activity was found by adding the supernatant from sonicated amoebocytes or hemocyte culture medium into spherulocyte preparations.
来自紫球海胆的体腔液含有多种体腔细胞类型，包括参与免疫防御的变形细胞和无色球状体细胞。在本文中，我们展示了体外检测的未分级体腔细胞对兔红细胞和K562肿瘤细胞系具有Ca(2+)依赖性细胞毒活性。在噬斑形成试验中，整个体腔细胞制剂以及密度梯度分离的体腔细胞群体显示，富含无色球状体细胞的细胞群体在变形细胞存在的情况下通过释放溶素发挥高细胞毒活性。这种协同效应可能依赖于变形细胞释放的可溶性因子。关于这一点，我们表明，通过将超声处理的变形细胞上清液或血细胞培养基添加到球状体细胞制剂中，发现细胞毒活性增强。
