Debernardi S, Skoulakis S, Molloy G, Chaplin T, Dixon-McIver A, Young B D
Medical Oncology Department, Cancer Research UK, Barts and the London School of Medicine, Queen Mary College, Charterhouse Square, London, UK.
Leukemia. 2007 May;21(5):912-6. doi: 10.1038/sj.leu.2404605. Epub 2007 Mar 1.
MicroRNAs (miRNAs) are short single-stranded RNAs that have a potentially important role in gene regulation. Using a quantitative real-time polymerase chain reaction assay specific to the mature miRNA, the expression level of a selected group of haematopoietic tissue-specific miRNAs was measured across a set of 30 primary adult acute myeloid leukaemia (AML) with a normal karyotype. The expression levels of each miRNA were correlated with the genome-wide mRNA expression profiles in the same leukaemias. This revealed that miR-181a correlated strongly with the AML morphological sub-type and with the expression of genes previously identified through sequence analysis as potential interaction targets. Three other miRNAs, miR-10a, miR-10b and miR-196a-1, showed a clear correlation with HOX gene expression.
微小RNA（miRNA）是短的单链RNA，在基因调控中可能发挥重要作用。使用针对成熟miRNA的定量实时聚合酶链反应分析，在一组30例核型正常的成人原发性急性髓系白血病（AML）中测量了一组选定的造血组织特异性miRNA的表达水平。每种miRNA的表达水平与相同白血病中的全基因组mRNA表达谱相关。这表明miR-181a与AML形态学亚型以及先前通过序列分析确定为潜在相互作用靶点的基因表达密切相关。其他三种miRNA，即miR-10a、miR-10b和miR-196a-1，与HOX基因表达呈现明显的相关性。
