• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种新型环状RNA circCSPP1通过海绵化miR-1182促进肝癌进展。

A Novel Circular RNA circCSPP1 Promotes Liver Cancer Progression by Sponging miR-1182.

作者信息

Jia Nan, Song Zhe, Chen Baosheng, Cheng Jinsheng, Zhou Wenyong

机构信息

Department of General Surgery, CangZhou General Hospital, CangZhou, Hebei, 061001, People's Republic of China.

出版信息

Onco Targets Ther. 2021 Apr 23;14:2829-2838. doi: 10.2147/OTT.S292320. eCollection 2021.

DOI:10.2147/OTT.S292320
PMID:33935503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8079351/
Abstract

INTRODUCTION

Aberrant circular RNA (circRNA) expression has been extensively discovered for its involvement in both the initiation and progression of various cancers. Through screening circRNA profile, we identified a novel circRNA has_circ_0001806, which is termed as circCSPP1 in liver cancer. In the present study, we aim to investigate the role of circCSPP1 in the progression of liver cancer.

METHODS

Fluorescence in situ hybridization (FISH) was used to detect the location of circCSPP1. Function studies including MTT, colony formation assay, transwell assay and flow cytometry were carried out to detect the malignant behaviour of circCSPP1 on liver cancer cells. Luciferase assay and RNA pull down were used to detect the interaction between miR-1182 and circCSPP1 as well as RAB15. Quantitative realtime (qPCR) and Western blot were performed to evaluate the RNA and protein expression, respectively.

RESULTS

CircCSPP1 knockdown inhibited the proliferation, migration and invasion while promoted apoptosis of liver cancer cells. Mechanically, we predicted and verified the target miR of circCSPP1 which is miR-1182. miR-1182 was capable of reversing the effect of circCSPP1 on liver cancer cells. Moreover, miR-1182 was found to also target RAB15 to participate in the regulation of cell phenotype.

DISCUSSION

Taken together, circCSPP1 promoted progression of liver cancer cells via sponging miR-1182 which may serve as a novel prognostic and therapeutic target for liver cancer.

摘要

引言

异常环状RNA（circRNA）的表达因其参与多种癌症的发生和发展而被广泛发现。通过筛选circRNA谱，我们鉴定出一种新型circRNA has_circ_0001806，在肝癌中它被命名为circCSPP1。在本研究中，我们旨在探讨circCSPP1在肝癌进展中的作用。

方法

采用荧光原位杂交（FISH）检测circCSPP1的定位。进行包括MTT、集落形成试验、Transwell试验和流式细胞术在内的功能研究，以检测circCSPP1对肝癌细胞恶性行为的影响。采用荧光素酶报告基因试验和RNA下拉试验检测miR-1182与circCSPP1以及RAB15之间的相互作用。分别进行定量实时（qPCR）和蛋白质免疫印迹法以评估RNA和蛋白质表达。

结果

circCSPP1敲低抑制了肝癌细胞的增殖、迁移和侵袭，同时促进了其凋亡。机制上，我们预测并验证了circCSPP1的靶标miR为miR-1182。miR-1182能够逆转circCSPP1对肝癌细胞的作用。此外，发现miR-1182也靶向RAB15参与细胞表型的调控。

讨论

综上所述，circCSPP1通过吸附miR-1182促进肝癌细胞进展，miR-1182可能成为肝癌新的预后和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/c15e12ac74b8/OTT-14-2829-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/330e2d881c71/OTT-14-2829-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/f65e73cd6826/OTT-14-2829-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/dcb76577249c/OTT-14-2829-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/3bf0cf5902a2/OTT-14-2829-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/ad7176bb6b38/OTT-14-2829-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/3bf1a16e5b5b/OTT-14-2829-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/c15e12ac74b8/OTT-14-2829-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/330e2d881c71/OTT-14-2829-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/f65e73cd6826/OTT-14-2829-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/dcb76577249c/OTT-14-2829-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/3bf0cf5902a2/OTT-14-2829-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/ad7176bb6b38/OTT-14-2829-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/3bf1a16e5b5b/OTT-14-2829-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faa3/8079351/c15e12ac74b8/OTT-14-2829-g0007.jpg

相似文献

1
A Novel Circular RNA circCSPP1 Promotes Liver Cancer Progression by Sponging miR-1182.一种新型环状RNA circCSPP1通过海绵化miR-1182促进肝癌进展。
Onco Targets Ther. 2021 Apr 23;14:2829-2838. doi: 10.2147/OTT.S292320. eCollection 2021.
2
Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1.环状 RNA circCSPP1 通过海绵吸附 miR-431 并调节 ROCK1 和 ZEB1 促进结肠癌的发生和发展。
J Transl Med. 2022 Jan 31;20(1):58. doi: 10.1186/s12967-022-03240-x.
3
Circular RNA circCSPP1 knockdown attenuates doxorubicin resistance and suppresses tumor progression of colorectal cancer via miR-944/FZD7 axis.环状RNA circCSPP1敲低通过miR-944/FZD7轴减弱多柔比星耐药性并抑制结直肠癌的肿瘤进展。
Cancer Cell Int. 2021 Mar 4;21(1):153. doi: 10.1186/s12935-021-01855-6.
4
Circular RNA circNELL2 Acts as the Sponge of miR-127-5p to Promote Esophageal Squamous Cell Carcinoma Progression.环状RNA circNELL2作为miR-127-5p的海绵促进食管鳞状细胞癌进展。
Onco Targets Ther. 2020 Sep 16;13:9245-9255. doi: 10.2147/OTT.S247847. eCollection 2020.
5
Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation.环状 RNA circCSPP1 通过抑制 miR-200c 的成熟来促进人骨肉瘤细胞的增殖并增加葡萄糖代谢。
Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221097364. doi: 10.1177/09603271221097364.
6
CircCSPP1 Functions as a ceRNA to Promote Colorectal Carcinoma Cell EMT and Liver Metastasis by Upregulating COL1A1.环状CSPP1作为一种竞争性内源RNA，通过上调COL1A1促进结肠癌细胞上皮-间质转化和肝转移。
Front Oncol. 2020 Jun 16;10:850. doi: 10.3389/fonc.2020.00850. eCollection 2020.
7
Circular RNA hsa_circ_0005397 promotes hepatocellular carcinoma progression by regulating the miR-326/PDK2 axis.环状RNA hsa_circ_0005397通过调控miR-326/PDK2轴促进肝细胞癌进展。
J Gene Med. 2021 Jun;23(6):e3332. doi: 10.1002/jgm.3332. Epub 2021 Apr 16.
8
Circular RNA circ_0003028 contributes to tumorigenesis by regulating GOT2 via miR-1298-5p in non-small cell lung cancer.环状 RNA circ_0003028 通过 miR-1298-5p 调控 GOT2 促进非小细胞肺癌的发生。
Bioengineered. 2021 Dec;12(1):2326-2340. doi: 10.1080/21655979.2021.1935064.
9
Exosomal circ_PIP5K1A regulates the progression of non-small cell lung cancer and cisplatin sensitivity by miR-101/ABCC1 axis.外泌体 circ_PIP5K1A 通过 miR-101/ABCC1 轴调控非小细胞肺癌的进展和顺铂敏感性。
Mol Cell Biochem. 2021 Jun;476(6):2253-2267. doi: 10.1007/s11010-021-04083-8. Epub 2021 Feb 11.
10
HnRNP-L-regulated circCSPP1/miR-520h/ axis modulates autophagy and promotes progression in prostate cancer.异质性核糖核蛋白L调控的环状CSPP1/微小RNA-520h轴调节自噬并促进前列腺癌进展。
Mol Ther Nucleic Acids. 2021 Oct 19;26:927-944. doi: 10.1016/j.omtn.2021.10.006. eCollection 2021 Dec 3.

引用本文的文献

1
Two-Dimensional-PAGE Coupled with nLC-MS/MS-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in MCF7 Breast Cancer Cells Transfected for JTB Protein Silencing.二维 PAGE 联合 nLC-MS/MS 鉴定 JTB 蛋白沉默转染 MCF7 乳腺癌细胞中的差异表达蛋白和肿瘤发生途径。
Molecules. 2023 Nov 9;28(22):7501. doi: 10.3390/molecules28227501.
2
CircUSP10 promotes liver cancer progression by regulating miR-211-5p/TCF12/EMT signaling pathway.环状USP10通过调控miR-211-5p/TCF12/EMT信号通路促进肝癌进展。
Heliyon. 2023 Oct 4;9(10):e20649. doi: 10.1016/j.heliyon.2023.e20649. eCollection 2023 Oct.
3

本文引用的文献

1
Circular RNA Sequencing Identifies CircASAP1 as a Key Regulator in Hepatocellular Carcinoma Metastasis.环状 RNA 测序鉴定 CircASAP1 为肝癌转移的关键调控因子。
Hepatology. 2020 Sep;72(3):906-922. doi: 10.1002/hep.31068.
2
CircularRNA as novel biomarkers in liver diseases.环状 RNA 作为肝脏疾病的新型生物标志物。
J Chin Med Assoc. 2020 Jan;83(1):15-17. doi: 10.1097/JCMA.0000000000000230.
3
CircRNA-5692 inhibits the progression of hepatocellular carcinoma by sponging miR-328-5p to enhance DAB2IP expression.circRNA-5692 通过海绵吸附 miR-328-5p 来抑制肝细胞癌的进展，从而增强 DAB2IP 的表达。
Circular RNAs: novel actors of Wnt signaling pathway in lung cancer progression.
环状RNA：肺癌进展中Wnt信号通路的新参与者
EXCLI J. 2023 Jul 12;22:645-669. doi: 10.17179/excli2023-6209. eCollection 2023.
4
Circ_0061395 functions as an oncogenic gene in hepatocellular carcinoma by acting as a miR-1182 sponge.环状 RNA 0061395 通过作为 miR-1182 的海绵体发挥致癌基因的作用在肝细胞癌中起作用。
Cell Cycle. 2022 Oct;21(20):2192-2205. doi: 10.1080/15384101.2022.2092177. Epub 2022 Jul 1.
5
Identification of circ_0000375 and circ_0011536 as novel diagnostic biomarkers of colorectal cancer.鉴定circ_0000375和circ_0011536作为结直肠癌的新型诊断生物标志物。
World J Clin Cases. 2022 Apr 16;10(11):3352-3368. doi: 10.12998/wjcc.v10.i11.3352.
6
The function and regulation network mechanism of circRNA in liver diseases.环状RNA在肝脏疾病中的功能及调控网络机制
Cancer Cell Int. 2022 Mar 31;22(1):141. doi: 10.1186/s12935-022-02559-1.
7
Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1.环状 RNA circCSPP1 通过海绵吸附 miR-431 并调节 ROCK1 和 ZEB1 促进结肠癌的发生和发展。
J Transl Med. 2022 Jan 31;20(1):58. doi: 10.1186/s12967-022-03240-x.
Cell Death Dis. 2019 Nov 27;10(12):900. doi: 10.1038/s41419-019-2089-9.
4
Plasma hsa_circ_0027089 is a diagnostic biomarker for hepatitis B virus-related hepatocellular carcinoma.血浆 hsa_circ_0027089 是乙型肝炎病毒相关肝细胞癌的诊断生物标志物。
Carcinogenesis. 2020 May 14;41(3):296-302. doi: 10.1093/carcin/bgz154.
5
Identification of two microRNA nodes as potential cooperative modulators of liver metabolism.鉴定两个作为肝脏代谢潜在协同调节因子的微小RNA节点。
Hepatol Res. 2019 Dec;49(12):1451-1465. doi: 10.1111/hepr.13419. Epub 2019 Sep 11.
6
Circular RNAs open a new chapter in cardiovascular biology.环状 RNAs 在心血管生物学中开辟了新篇章。
Nat Rev Cardiol. 2019 Aug;16(8):503-514. doi: 10.1038/s41569-019-0185-2.
7
The Landscape of Circular RNA in Cancer.环状 RNA 在癌症中的研究进展
Cell. 2019 Feb 7;176(4):869-881.e13. doi: 10.1016/j.cell.2018.12.021.
8
Widespread and Functional RNA Circularization in Localized Prostate Cancer.局限性前列腺癌中广泛存在和功能性的 RNA 环化。
Cell. 2019 Feb 7;176(4):831-843.e22. doi: 10.1016/j.cell.2019.01.025.
9
The circular RNome of primary breast cancer.原发性乳腺癌的环状 RNA 组。
Genome Res. 2019 Mar;29(3):356-366. doi: 10.1101/gr.238121.118. Epub 2019 Jan 28.
10
Endometrial Epithelial Cell Apoptosis Is Inhibited by a ciR8073-miR181a-Neurotensis Pathway during Embryo Implantation.在胚胎植入过程中，子宫内膜上皮细胞凋亡受到ciR8073-miR181a-神经降压素通路的抑制。
Mol Ther Nucleic Acids. 2019 Mar 1;14:262-273. doi: 10.1016/j.omtn.2018.12.005. Epub 2018 Dec 14.