Didenko Vladimir V, Minchew Candace L, Shuman Stewart, Baskin David S
Baylor College of Medicine, Houston, Texas, Michael E. DeBakey Veterans Affairs Medical Center, Houston, Texas, and Sloan-Kettering Institute, New York, New York.
Nano Lett. 2004 Dec;4(12):2461-6. doi: 10.1021/nl048357e.
The design of artificial molecular machines is complicated because the mechanics used in macromachines is not readily adaptable for nano environments. We constructed a semi-artificial molecular device, which contains a naturally occurring molecular machine-a vaccinia virus encoded protein-linked with an artificial part. The self-assembled construct makes two fluorescently labeled detector units. It is the first sensor capable of selectively detecting different types of DNA breaks, exemplifying a practical approach to the design of molecular devices.
人工分子机器的设计很复杂，因为大分子机器中使用的力学原理不易适用于纳米环境。我们构建了一种半人工分子装置，它包含一个天然存在的分子机器——一种与人工部分相连的痘苗病毒编码蛋白。这种自组装结构形成了两个荧光标记的检测单元。它是第一个能够选择性检测不同类型DNA断裂的传感器，为分子装置的设计提供了一种实用方法。
