福尔马林固定组织切片中DNase II型断裂的快速检测

Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections.

作者信息

Minchew Candace L, Didenko Vladimir V

机构信息

Department of Neurosurgery, Baylor College of Medicine, Houston, TX, USA.

Michael E. DeBakey Veterans Affairs Medical Center, 2002 Holcombe Boulevard, Research Building 109, Room 204, Houston, TX, 77030, USA.

出版信息

Methods Mol Biol. 2017;1644:113-119. doi: 10.1007/978-1-4939-7187-9_9.

DOI:10.1007/978-1-4939-7187-9_9

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5654645/

Abstract

Blunt-ended DNase II-type breaks with 5' hydroxyls are generated in phagocytic cells of any lineage during digestion of the engulfed DNA. These breaks indicate the ongoing active phagocytic reaction. They are produced by the acid deoxyribonuclease-DNase II which is the primary endonuclease responsible for DNA degradation after its engulfment.Here, we present an express approach that detects blunt-ended 5' OH DNA breaks in fixed tissue sections. The technique is simple to perform and takes only 60 min to complete. It can be useful in studies of the clearance of dying cells in oncological, inflammatory, and autoimmune disorders.

摘要

在吞噬细胞吞噬DNA的消化过程中，会产生带有5'羟基的平端DNase II型断裂。这些断裂表明正在进行的活跃吞噬反应。它们是由酸性脱氧核糖核酸酶-DNase II产生的，该酶是吞噬后负责DNA降解的主要内切核酸酶。在此，我们提出一种在固定组织切片中检测平端5' OH DNA断裂的快速方法。该技术操作简单，仅需60分钟即可完成。它可用于肿瘤学、炎症和自身免疫性疾病中死亡细胞清除的研究。

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