Azanza M J, Pes N, Pérez-Bruzón R N, Aisa J, Raso M, Junquera C, Lahoz J M, Maestú C, Martínez-Ciriano C, Pérez-Castejón C, Vera-Gil A, Del Moral A
Laboratorio de Magnetobiología, Departamento de Anatomía e Histología Humanas, Facultad de Medicina, Universidad de Zaragoza, Zaragoza, Spain.
Histol Histopathol. 2007 May;22(5):497-504. doi: 10.14670/HH-22.497.
The aim of the present study was to examine the distribution of cells expressing connexin 26 (Cx26) in the suboesophageal visceral, left and right parietal and left and right pleural ganglia of the snail Helix aspersa by immunocytochemistry. Altogether we have found approximately 452 immunoreactive neurons which represent the 4.7% of the total neurons counted. The stained large neurons (measured diameter 55-140 microm) occurred mostly on the peripheral surface of the ganglia while the small immunostained cells (5-25 microm diameter) were observed in groups near the neuropil. The number of large neurons giving positive Cx26-like immunostaining was small in comparison with that for medium (30-50 microm diameter) and small sized cells. The expression of Cx26 was also observed in the processes of glia cells localized among neurons somata and in the neuropil showing that the antiserum recognized epitopes in both protoplasmic and fibrous glia cells of Helix aspersa. The neuropils of all ganglia showed fibers densely immunostained. While we have observed a good specificity for Cx26-antiserum in neurons, a lack of reaction for Cx43 antiserum was observed in neurons and glia cells. The reaction for enolase antiserum in neurons was light and non-specific and a lack of reaction in glia cells and processes for GFAP antiserum was observed. Although the percentage of positive neurons for Cx26 antiserum was low is suggested that in normal physiological conditions or under stimulation the expression of connexin could be increased. The observed results can be considered of interest in the interpretation of Helix aspersa elemental two neuron networks synchronizing activity, observed under applied extremely low frequency magnetic fields.
本研究的目的是通过免疫细胞化学方法检测表达连接蛋白26（Cx26）的细胞在蜗牛（Helix aspersa）食管下内脏神经节、左右壁神经节以及左右胸膜神经节中的分布情况。我们总共发现了约452个免疫反应性神经元，占所计数神经元总数的4.7%。染色的大神经元（测量直径为55 - 140微米）大多出现在神经节的外周表面，而小的免疫染色细胞（直径5 - 25微米）则成组地出现在神经纤维网附近。与中等大小（直径30 - 50微米）和小尺寸的细胞相比，呈现Cx26样免疫染色阳性的大神经元数量较少。在位于神经元胞体之间和神经纤维网中的神经胶质细胞的突起中也观察到了Cx26的表达，这表明抗血清识别了Helix aspersa原生质和纤维性神经胶质细胞中的表位。所有神经节的神经纤维网都显示出密集免疫染色的纤维。虽然我们在神经元中观察到Cx26抗血清具有良好的特异性，但在神经元和神经胶质细胞中未观察到Cx43抗血清的反应。神经元中烯醇化酶抗血清的反应较弱且非特异性，并且在神经胶质细胞及其突起中未观察到对GFAP抗血清的反应。尽管Cx26抗血清阳性神经元的百分比很低，但提示在正常生理条件下或受到刺激时，连接蛋白的表达可能会增加。在解释在施加极低频磁场时观察到的Helix aspersa基本双神经元网络同步活动方面，所观察到的结果可能具有一定意义。
