Kerem Mustafa, Bedirli Abdulkadir, Pasaoglu Hatice, Unsal Cigdem, Yilmaz Tonguc Utku, Ofluoglu Ebru, Sahin Tevfik Tolga
Department of General Surgery, Gazi University, Medical Faculty, 06510 Besevler, Ankara, Turkey.
Dig Dis Sci. 2007 Apr;52(4):950-5. doi: 10.1007/s10620-006-9150-0. Epub 2007 Feb 27.
Ghrelin and leptin are the hormones that influence endocrine and exocrine functions of the pancreas and regulate feeding behaviors and energy metabolism. The aim of this study was to investigate the levels of ghrelin and leptin in pancreatitis of different severities and the relation of these hormones with blood glucose level and proinflammatory cytokines. The study was performed on 90 Wistar Albino rats. Three experimental groups composed of 30 rats were established: control group, 0.9% NaCl solution was injected intraperitoneally (i.p); acute edematous pancreatitis (AEP) group, 1 microg/100 g cerulein was injected i.p. five times, at 1-hr intervals; and acute necrotizing pancreatitis (ANP) group, 500 mg/100 g L-arginine was injected i.p. Ten animals in each group were sacrificed under anesthesia 12, 24 and 48 hr after the last injection. After blood withdrawal, the pancreas was totally excised. The levels of blood sugar, lipase, serum tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), ghrelin, and leptin were investigated and histopathologic examination was performed. Following pancreatitis induction, serum ghrelin levels increased at 24 hr and reached the peak level at 48 hr. Its level in the AEP group was higher than in the ANP group. Serum leptin levels in the AEP and ANP groups increased after 12 hr and stayed at high levels until 48 hr compared with the control group. Similarly to ghrelin and leptin, blood glucose levels increased in both pancreatitis groups, but the increase was more prominent in the ANP group, with levels >200 mg/ml at 48 hr. The levels of TNF-alpha and IL-1beta in the AEP and ANP groups reached the peak level at 24 hr and then decreased to a level close to that of the control group at 48 hr. We conclude that serum leptin and ghrelin levels increase in the first 48 hr of AEP and ANP. As the serum ghrelin levels in ANP are higher than in AEP, it can be used as a marker to show the severity of pancreatitis. While TNF-alpha and IL-1beta can be used as a prognostic factor in the first 24 hr, ghrelin and leptin can be used subsequently.
胃饥饿素和瘦素是影响胰腺内分泌和外分泌功能、调节进食行为和能量代谢的激素。本研究旨在调查不同严重程度胰腺炎患者的胃饥饿素和瘦素水平，以及这些激素与血糖水平和促炎细胞因子之间的关系。该研究以90只Wistar白化大鼠为对象。建立了由30只大鼠组成的三个实验组：对照组，腹腔注射0.9%氯化钠溶液；急性水肿性胰腺炎（AEP）组，每隔1小时腹腔注射1微克/100克雨蛙素，共注射5次；急性坏死性胰腺炎（ANP）组，腹腔注射500毫克/100克L-精氨酸。每组10只动物在最后一次注射后12、24和48小时麻醉状态下处死。采血后，完整切除胰腺。检测血糖、脂肪酶、血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、胃饥饿素和瘦素水平，并进行组织病理学检查。诱导胰腺炎后，血清胃饥饿素水平在24小时时升高，并在48小时时达到峰值水平。其在AEP组中的水平高于ANP组。与对照组相比，AEP组和ANP组的血清瘦素水平在12小时后升高，并在48小时内保持在高水平。与胃饥饿素和瘦素类似，两个胰腺炎组的血糖水平均升高，但在ANP组中升高更为显著，48小时时血糖水平>200毫克/毫升。AEP组和ANP组的TNF-α和IL-1β水平在24小时时达到峰值，然后在48小时时降至接近对照组的水平。我们得出结论，在AEP和ANP的最初48小时内，血清瘦素和胃饥饿素水平升高。由于ANP中的血清胃饥饿素水平高于AEP，它可作为显示胰腺炎严重程度的标志物。虽然TNF-α和IL-1β可在最初24小时用作预后因素，但胃饥饿素和瘦素随后可使用。
