从活性污泥中分离得到的戈登氏菌（Gordonia sp.）菌株 JDC-2 和节杆菌（Arthrobacter sp.）菌株 JDC-32 通过生化协同作用完全降解邻苯二甲酸二辛酯。
Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge.
机构信息
Department of Bioengineering, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
出版信息
J Hazard Mater. 2010 Apr 15;176(1-3):262-8. doi: 10.1016/j.jhazmat.2009.11.022. Epub 2009 Nov 10.
Two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (PAEs) as the sole source of carbon and energy. One of the isolates was identified as Gordonia sp. strain JDC-2 and the other as Arthrobacter sp. strain JDC-32, mainly through 16S rRNA gene sequence analysis. Gordonia sp. strain JDC-2 rapidly degraded di-n-octyl phthalate (DOP) into phthalic acid (PA), which accumulated in the culture medium. Arthrobacter sp. strain JDC-32 degraded PA but not DOP. The co-culture of Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 degraded DOP completely by overcoming the degradative limitations of each species alone. The biochemical pathway of DOP degradation by Gordonia sp. strain JDC-2 was proposed based on the identified degradation intermediates. The results suggest that DOP is completely degraded by the biochemical cooperation of different microorganisms isolated from activated sludge.
从含有邻苯二甲酸酯（PAEs）的混合溶液中作为唯一碳源和能源，分离出两株细菌菌株。其中一株被鉴定为戈登氏菌（Gordonia）菌株 JDC-2，另一株被鉴定为节杆菌（Arthrobacter）菌株 JDC-32，主要通过 16S rRNA 基因序列分析。戈登氏菌（Gordonia）菌株 JDC-2 可迅速将邻苯二甲酸二辛酯（DOP）降解为邻苯二甲酸（PA），并在培养基中积累。节杆菌（Arthrobacter）菌株 JDC-32 可降解 PA，但不能降解 DOP。通过克服每种单独的降解限制，戈登氏菌（Gordonia）菌株 JDC-2 和节杆菌（Arthrobacter）菌株 JDC-32 的共培养可以完全降解 DOP。基于鉴定的降解中间产物，提出了戈登氏菌（Gordonia）菌株 JDC-2 降解 DOP 的生化途径。结果表明，从活性污泥中分离出的不同微生物通过生化合作可完全降解 DOP。
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